| 白细胞介素-10对实验性肝纤维化大鼠基质金属蛋白酶抑制剂-1,-2的影响 |
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| 引用本文： | 黄月红,张莉娟,陈运新,陈治新,王小众.白细胞介素-10对实验性肝纤维化大鼠基质金属蛋白酶抑制剂-1,-2的影响[J].福建医科大学学报,2003,37(3):244-246,F002. |
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| 作者姓名： | 黄月红 张莉娟 陈运新 陈治新 王小众 |
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| 作者单位： | 福建医科大学,附属协和医院消化内科,福州,350001 |
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| 基金项目： | 福建省科技厅科技开发计划项目 ( 2 0 0 3 D0 5 ) |
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| 摘 要： | 目的 研究白细胞介素 - 10 (IL- 10 )对实验性肝纤维化大鼠基质金属蛋白酶抑制剂 - 1,- 2 (TIMP- 1,- 2 )的影响。 方法 建立大鼠肝纤维化模型并行 IL- 10干预实验 ,从正常大鼠 (N组 )和 CCl4 诱导肝纤维化大鼠 (C组 )及 IL- 10干预肝纤维化大鼠 (E组 )中取肝脏组织 ,SP免疫组织化学法检测各组大鼠肝脏组织中 TIMP- 1,- 2的表达。 结果 肝脏组织病理学证实 ,成功构建 CCl4 诱导的实验性大鼠肝纤维化模型 ,随注射 CCl4 次数增加 ,肝纤维化程度逐渐加重。TIMP- 1阳性染色多见于大鼠肝细胞的细胞质 ,汇管区的胆管细胞细胞质有少许阳性表达。C组较 N组 TIMP- 1阳性表达明显增强 ,经 Ridit分析 ,差异有显著性 (P<0 .0 5 ) ;且 TIMP- 1随着纤维化的发展表达逐渐增加 (P<0 .0 5 )。E组阳性表达较 C组降低 (P<0 .0 5 )。TIMP- 2阳性染色多见于大鼠肝细胞的细胞质 ,C组和E组较 N组的 TIMP- 2阳性表达差异有显著性 (P<0 .0 5 ) ,但 C组和 E组差异无显著性 (P>0 .0 5 )。 结论 TIMP- 1随着大鼠肝纤维化进展表达逐渐升高 ,IL- 10通过减少 TIMP- 1的表达 ,有抗纤维化作用
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| 关 键 词： | 白细胞介素10 金属蛋白酶类组织抑制剂 肝硬化 免疫组织化学 大鼠 |
| 文章编号： | 1672-4194(2003)03-0244-03 |
Effect of Interleukin-10 on Expression of Metalloproteinase Inhibitor-1, -2 in Experimental Liver Fibrosis Rat |
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| HUANG Yue\|hong,ZHANG Li\|juan,CHEN Yun\|xin,CHEN Zhi\|xin,WANG Xiao\|zhong.Effect of Interleukin-10 on Expression of Metalloproteinase Inhibitor-1, -2 in Experimental Liver Fibrosis Rat[J].Journal of Fujian Medical University,2003,37(3):244-246,F002. |
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| Authors: | HUANG Yue\|hong ZHANG Li\|juan CHEN Yun\|xin CHEN Zhi\|xin WANG Xiao\|zhong |
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| Affiliation: | HUANG Yue\|hong,ZHANG Li\|juan,CHEN Yun\|xin,CHEN Zhi\|xin,WANG Xiao\|zhong Department of Gastroenterology,The Affiliated Union Hospital,Fujian Medical University,Fuzhou\ 350001,China |
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| Abstract: | Objective\ To investigate the effect of interleukin\|10(IL\|10) on expression of tissue inhibitor of metalloproteinase\|1,\|2(TIMP\|1,\|2) in experimental rat liver fibrosis.\ Methods\ Animal model of liver fibrosis and IL\|10 intervention model were developed in SD rats.\ To measured and analysed expression of metalloproteinase inhibitor\|1 and metalloproteinase inhibitor\|2 in liver tissue from normal rats(group N), CCl\-4 induced liver fibrosis rats(group C), and IL\|10 pretreated rats(group E) respectively.\ Results\ The CCl\-4\|induced experimental rat hepatic fibrosis model was successfully established and liver fibrosis was developed in 74 SD rats.\ TIMP\|1 was positively expressed often in cytoplasm of hepatocytes and rarely in cytoplasm of biliary epithelial cells in portal area.\ Redit analysis indicated that the positive expression of TIMP\|1 in group C increased remarkly than of group N, and the increase was parallel to the progression of liver fibrosis; the positive expression in group E was significantly stronger than that in group C(P<0 05).\ The positive expression of TIMP\|2 was frequently found in cytoplasm of hepatocytes, the positive expression in group C and E was stronger than that in group N(P<0 05), but there was no significant difference between group C and E.\ Conclusion\ With the progression of the liver fibrosis, the expression of TIMP\|1, \|2 increased gradually; the antifibrotic mechanism of IL\|10 partly due to its downregulation on the expression of TIMP\|1 |
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| Keywords: | interleukin\|10 tissue inhibitor of metalloproteinase liver fibrosis immunohistochemistry rat |
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