龙眼DCL4的亚细胞定位分析及其对外源ABA和SA的响应

为了研究DlDCL家族中DlDCL4基因在龙眼中的功能,本研究通过氨基酸序列比对、系统发育进化树的构建、亚细胞定位验证、实时荧光定量PCR（qPCR）等方法,对其进行初步探究。结果表明：遗传进化分析显示,DCL4氨基酸序列在所选的物种间相似度高于50%,其中龙眼与甜橙（Citrus sinensis）的亲缘关系最近。在激光共聚焦显微镜观察下,洋葱细胞核发出绿色荧光,其他部位不发出荧光,表明龙眼DCL4蛋白定位于细胞核中。在24 h内,100 μmol/L的外源脱落酸（ABA）对 DlDCL4都有极显著的下调效果;100 μmol/L的水杨酸（SA）对其表达量有显著的上调效果,其中8 h时表达量最大,提示 DlDCL4对不同激素的响应效果不同且不同处理时长下响应效果也会有影响。

Subcellular Localization Analysis and Responses to Exogenous ABA and SA in Longan Callus of DCL4

This study carried out amino acid sequence alignment, phylogenetic tree analysis, subcellular localization verification and real-time qPCR analysis to study the function of DlDCL4 gene of DlDCL family in longan. It was found that the similarity of the amino acid sequence of DCL4 was more than 50% in the selected species. In the evolutionary relationship, DlDCL4 and navel orange (Citrus sinensis) were closely related. Under the observation of laser confocal microscopy, the onion cell nucleus emitted green fluorescence, and other parts did not emit fluorescence, so the longan DCL4 protein was localized in the nucleus. In 24 h, 100 μmol/L exogenous ABA hormone had a significant down-regulation effect on DlDCL4. SA had a significant up-regulation effect on its expression, and the effect was higher than other time treatment at 8 h, which suggested thatDlDCL4 had different responses to different hormones and this effect would also be affected by different treatment durations.