维生素E对老年大鼠卵巢颗粒细胞保护作用机制的探讨

目的：通过观察维生素E（VE）对老年雌性大鼠卵巢凋亡相关蛋白Bcl-2、Bax的影响及对其抗氧化能力的影响,探讨VE延缓卵巢衰老的作用和机制。方法：采用自然衰老雌性大鼠,给予不同剂量外源性VE,用免疫组化方法观察卵巢颗粒细胞凋亡调控蛋白Bcl-2、Bax的表达,用Western Blot法检测卵巢Bcl-2、Bax蛋白含量,用生化法测定血清总超氧化物歧化酶（SOD）活力和丙二醛（MDA）含量。结果：与成年对照组相比,老年对照组Bcl-2表达明显降低、Bax表达明显升高（P〈0．01）,血清中SOD活力下降、MDA含量显著升高（P〈0．01）。与老年对照组相比,VE纽Bcl-2表达升高,Bax表达降低（P〈0．05）,MDA含量显著减少（P〈0．01）。结论：VE可调控凋亡相关蛋白Bcl-2、Bax的表达和对抗自由基对颗粒细胞的损伤,对卵巢颗粒细胞具有一定的保护作用。

Study on protective effect of vitamin E for ovarian grandlose cells and its mechanism in aged rats

Aim： To bserve the effect of vitamin E （VE） on ovarian apoptosis-related protein Bcl-2 and Bax and its impact on antioxidant capacity in aged female rats and to study the senility-delaying effect and mechanism of VE on ovary. Methods： Natural aging female rats were given different doses of exogenous VE. Then apoptosis regulatory protein Bcl-2, Bax expression in ovarian grandiose cells were detected by using immtmohistochemical methods and Western blot. The contents of serum total superoxide dismutase（SOD） activity and malondialdehyde（MDA） were detected by using biochemical methods. Results： Contrasted with adult control group, the level of Bcl-2 expression in Senile control group was lower and the level of Bax expression was higher（ P 〈 0.01）, Serum SOD activity decreased and the level of MDA significantly increased （ P 〈 0.01）. Contrasted with senile control group , the level of Bcl-2 expression increased in VE group, the level of Bax expression decreased （P 〈 0.05）, the level of MDA expression significantly decreased（ P 〈 0.01 ）. Conclusion： VE can regulate apoptosis-related protein Bcl-2, Bax expression and confront free radical damage which contribute to a protective effect for ovarian grandiose cells.