实时荧光核酸恒温扩增技术检测尿液中沙眼衣原体的分析

目的评价实时荧光核酸恒温扩增技术（SAT）检测尿液中沙眼衣原体（CT）的特异性和敏感性。方法对175例疑似患者生殖道拭子行CT—SAT、PCR检测和沙眼衣原体培养，同时对对应的175份尿液标本行CT—SAT检测。结果SAT对同一患者的拭子标本和尿液标本的检测结果符合率100％。1例淋球菌培养阴性而SAT阳性，2例PCR阳性而SAT阴性。结论SAT技术检测拭子及尿液中的沙眼衣原体具有很高的灵敏度和特异性，为CT的实验室诊断提供新的检测方法。

Real-time Fluorescence Detection of Nucleic Acid Amplification Constant Urine Analysis of Chlamydia Trachomatis

Objective To evaluate the real-time isothermal nucleic acid amplification fluorescence （SAT） Chlamydia trachomatis in urine （ CT ） of the specificity and sensitivity. Methods 175 cases of suspected patients of genital swab line CT-SAT, PCR testing and Chlamydia trachomatis culture, while the corresponding urine specimens of 175 CT-SAT test. Results SAT on the same source swab specimens and urine samples consistent results： Case of SAT-positive and N.gonorrhoeae culture-negative,2 SAT-negative and PCR positive. Conclusion SAT swabs and urine detection of Chlamydia trachomatis with high sensitivity and specificity of laboratory diagnosis for the CT to provide a new detection method.