| 利用PCR技术快速检测玉米细菌性枯萎病菌(Pantoea stewartii subsp.stewartii) |
| |
| 引用本文： | 吴兴海,杨伟克,邓明俊,陈长法,邵秀玲,梁成珠,封立平. 利用PCR技术快速检测玉米细菌性枯萎病菌(Pantoea stewartii subsp.stewartii)[J]. 甘肃农业大学学报, 2007, 42(5): 88-91 |
| |
| 作者姓名： | 吴兴海 杨伟克 邓明俊 陈长法 邵秀玲 梁成珠 封立平 |
| |
| 作者单位： | 山东出入境检验检疫局,山东,青岛,266002 |
| |
| 基金项目： | 国家质检总局植物病原检测专项基金(2005IK066) |
| |
| 摘 要： | 根据玉米细菌性枯萎病菌(Pantoea stewartiisubsp.stewartii)ITS基因序列,设计并合成了特异性PCR检测引物,对玉米细菌性枯萎病菌和非玉米细菌性枯萎病菌的标准菌株进行了PCR扩增反应.结果显示,玉米细菌性枯萎病菌的PCR产物出现301 bp的特异性扩增条带,而非玉米细菌性枯萎病菌均未出现扩增条带;其PCR检测的灵敏度为612 pg/μL.因此PCR技术是一种特异、灵敏、快速检测玉米细菌性枯萎病菌的方法.
|
| 关 键 词： | PCR 玉米细菌性枯萎病菌 检测 |
| 文章编号： | 1003-4315（2007）05-0088-04 |
| 修稿时间： | 2006-11-12 |
Application of PCR method in rapid identification of Pantoea stewartii subsp, stewartii |
| |
| WU Xing-hai,YANG Wei-ke,DENG Ming-jun,CHEN Chang-fa,SHAO Xiu-ling,LIANG Cheng-zhu,FENG Li-ping. Application of PCR method in rapid identification of Pantoea stewartii subsp, stewartii[J]. Journal of Gansu Agricultural University, 2007, 42(5): 88-91 |
| |
| Authors: | WU Xing-hai YANG Wei-ke DENG Ming-jun CHEN Chang-fa SHAO Xiu-ling LIANG Cheng-zhu FENG Li-ping |
| |
| Affiliation: | Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002 ,China |
| |
| Abstract: | A rapid identification method based on molecular biology of Pantoea stewartii subsp.stewartii was established by using one pair of primers unique to ITS(16 S~23 S rRNA interspacer region) gene segments.A specific DNA amplicon was present in the standard strains,but absent in the non-stand strains,which showed that one pair of primers are a rapid molecular biological identification method of Pantoea stewartii subsp.stewartii.The sensitivity of this method was confirmed by serial dilutions while approximately 104,and the level of detection of the primer was 612 pg/μL DNA solution. |
| |
| Keywords: | PCR Pantoea stewartii subsp.stewartii identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
