益气温阳法联合顺铂对荷瘤小鼠调节性T细胞的影响

目的:研究益气温阳法联合顺铂对Lewis肺癌小鼠调节性T(T Regulatory,Treg)细胞的影响。方法:建立40只近交系C57BL/6纯系雄性小鼠Lewis肺癌细胞皮下移植瘤模型后,将40只造模后的小鼠随机分为模型组、中药组、顺铂组及联合用药组,每组10只,单笼饲养。模型组以0.4 mL生理盐水连续灌胃12 d;中药组以0.4 mL中药液连续灌胃12 d(20 g生药/kg);顺铂组以0.2 mL顺铂溶液腹腔注射,分别在第1天、第3天、第5天腹腔注射顺铂溶液(4 mg顺铂/kg);联合用药组以0.4 mL中药液连续灌胃12 d(20 g生药/kg),在第1天、第3天、第5天腹腔注射顺铂溶液0.2 mL(4 mg顺铂/kg)。给予药物干预12 d后,用流式细胞术检测各组小鼠脾脏组织Treg细胞的比例。结果:流式细胞术结果表明,益气温阳法治疗可显著降低Lewis肺癌小鼠脾脏组织Treg细胞,与模型组比较,中药组、顺铂组和联合用药组小鼠脾脏组织内CD4+CD25+Treg细胞比例与CD4+CD25+Foxp3+Treg细胞比例均显著降低(P<0.05)。结论:益气温阳法通过降低Treg细胞的水平增强机体的抗肿瘤免疫应答而发挥抑瘤作用。

Effects of Yiqi Wenyang Combined with Cisplatin on Regulatory T cells of Lewis Lung Cancer Mice

To study the effects of Yiqi Wenyang method combined with cisplatin on T regulatory cell (Treg) of Lewis lung cancer mice.Methods:After 40 inbred C57BL/6 male mice with Lewis lung cancer cell transplanted subcutaneously were established,They were randomly divided into model group,traditional Chinese medicine group,cisplatin group and combined drug group,10 in each group,fed in single cage.The model group was given 0.4 mL saline for 12 days;In the traditional Chinese medicine group,0.4 mL of traditional Chinese medicine solution was infused into the stomach for 12 days (20 g crude drug/kg);In cisplatin group,0.2 mL cisplatin solution was intraperitoneally injected,and cisplatin solution (4 mg cisplatin/kg) was intraperitoneally injected on the first day,the third day and the fifth day respectively;In the combined group,0.4 mL traditional Chinese medicine solution was infused into the stomach for 12 days (20 g crude drug/kg),and 0.2 mL cisplatin solution (4 mg cisplatin/kg) was injected intraperitoneally on the first day,the third day and the fifth day.After 12 days of drug intervention,the proportion of Treg cells in spleen tissue of mice in each group was detected by flow cytometry.Results:the results of flow cytometry showed that the treatment of Yiqi Wenyang method could significantly reduce the spleen Treg cells of Lewis lung cancer mice.Compared with the model group,the number of the proportion of CD4+CD25+ Treg cells and the proportion of CD4+CD25+Foxp3+ Treg cells in spleen tissue of mice in the traditional Chinese medicine group,cisplatin group and combination group were significantly reduced (P<0.05).Conclusion:the method of Supplementing Qi and warming yang can inhibit tumor by reducing the level of Treg cells and enhancing the anti-tumor immune response.