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| 水稻白叶枯病抗性基因Xa23鉴别菌株突变体库的构建及无毒基因突变体的筛选 | |
| 引用本文： | 周永力,潘雅姣,翟文学,徐建龙,章琦,黎志康.水稻白叶枯病抗性基因Xa23鉴别菌株突变体库的构建及无毒基因突变体的筛选[J].生物工程学报,2005,21(3):486-488. |
| 作者姓名： | 周永力 潘雅姣 翟文学 徐建龙 章琦 黎志康 |
| 作者单位： | 1. 中国农业科学院作物科学研究所,农业部作物遗传育种重点实验室,北京,100081 2. 中国农业科学院作物科学研究所,农业部作物遗传育种重点实验室,北京,100081;沈阳农业大学生物技术学院,沈阳,110161 3. 中国科学院遗传与发育生物学研究所,北京,100101 |
| 摘 要： | 利用in vivo转座技术构建了白叶枯病抗性基因Xa23鉴别菌株的突变体库，特异性引物PCR扩增和转座子插入位点旁侧序列分析结果表明转座子插入到白叶枯病菌的基因组中。经人工接种鉴定，筛选到4个毒力发生变化的突变体。为进一步克隆Xa23无毒基因提供了条件。 |
| 关 键 词： | Xanthonmonas oryzae pv oryzae，Xa23，突变体 |
| 文章编号： | 1000-3061(2005)03-0486-03 |
| 修稿时间： | 2004年10月25 |
Construction of Mutant Population of Differential Race of Xa23 Resistant to Rice Bacterial Blight and Avirulence Activity Identification of Mutants |
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| ZHOU Yong-li,PAN Ya-Jiao,ZHAI Wen-Xue,XU Jian-long,ZHANG Qi,LI Zhi-kang.Construction of Mutant Population of Differential Race of Xa23 Resistant to Rice Bacterial Blight and Avirulence Activity Identification of Mutants[J].Chinese Journal of Biotechnology,2005,21(3):486-488. | |
| Authors: | ZHOU Yong-li PAN Ya-Jiao ZHAI Wen-Xue XU Jian-long ZHANG Qi LI Zhi-kang |
| Affiliation: | Key Laboratory of Crop Genetic and Breeding of Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China. |
| Abstract: | The mutant population of Xanthomonas oryzae pv oryzae strain differential to rice bacterial blight resistance gene Xa23 has been constructed mediated by transposon in vivo. The results of PCR amplification with specific primers and analysis of flanking sequence of mutants indicated that the foreign DNA has been integrated into X. oryzae pv oryzae genome. Four mutants with changed avirulent activity to Xa23 gene have been identified by artificial inoculation. It is possible to clone genes that are required for AvrXa23 avirulence activity using this new strategy. |
| Keywords: | Xanthonmonas oryzae pv oryzae Xa23 mutant |
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