HPLC法测定复方黄酮胶囊的溶出度

目的:建立以高效液相色谱法测定复方黄酮胶囊溶出度的方法。方法:色谱柱为Nova-Pak C18(250mm×4.0mm,5μm),流动相为甲醇-0.4%磷酸(50:50),流速为1.0mL.min-1,检测波长为360nm,柱温为30℃。溶出度测定采用转篮法,以0.1mol.L-1盐酸为溶剂,转速为100r.min-1。结果:胶囊的累积溶出度在30min后均大于80%。槲皮素进样量在0.06584～0.6584μg范围内与峰面积积分值呈良好线性关系(r=0.9999);平均加样回收率为99.35%,RSD=0.92%(n=6)。结论:本方法简便、准确、重现性好,可用于复方黄酮胶囊的质量控制。

Determination of the Dissolution of Compound Flavone Capsule by HPLC

OBJECTIVE:To determinate the dissolution of Compound Flavone capsules by HPLC.METHODS:Nova-Pak C18(250mm× 4.0mm,5μ m) column was used with column at room temperature.The mobile phase consisted of methanol-0.4% H3PO4(50:50) at a flow rate of 1.0mL· min-1.The detective wavelength was 360nm.The dissolution of the Compound Flavone capsules was determined by basket stirring technique with 0.1mol· L-1 hydrochloric acid as dissolvent at a speed of 100r· min-1.RESULTS:The cumulative dissolution rate of Compound Flavone capsules was above 80% at 30 minutes.The linear range of Quercetin was 0.065 84～ 0.658 4μg(r=0.999 9).The average recovery was 99.35%,RSD=0.92%(n=6).CONCLUSION:The method is simple,accurate and reproducible,and suitable for the guality control of Compound Flavone capsules.