一测多评法结合指纹图谱在枳壳质量评价中的应用

目的基于一测多评(QAMS)与指纹图谱的方法建立27批枳壳的质量评价方法。方法以柚皮苷为参照,计算芸香柚皮苷、橙皮苷、新橙皮苷的相对校正因子,并测定其含量,比较外标法与QAMS法的差异性。采用HPLC法,建立27批枳壳饮片的指纹图谱,通过聚类分析(cluster analysis,CA)、主成分分析(principal component analysis,PCA)对指纹图谱进行评价。结果QAMS法与外标法测量值之间无显著性差异;建立枳壳饮片指纹图谱方法,相似度均>0.95,共标定11个共有峰,通过与对照品谱图比对,确定了3号峰为芸香柚皮苷、4号峰为柚皮苷、5号峰为橙皮苷、6号峰为新橙皮苷。结论QAMS法结合指纹图谱可为评价枳壳饮片的质量提供参考。

Application of fingerprint combined with QAMS in quality evaluation of Aurant Fructus

Objective To establish the quality evaluation method of 27 batches of Aurantii Fructus based on QAMS and the fingerprint. Methods Taking naringin as the reference, the relative correction factors of narirutin, hesperidin, and neohesperidin were calculated. The contents were determined and the differences between the external standard method and QAMS method were compared. And the fingerprints of 27 batches of decoction pieces of Aurantii Fructus were established by HPLC, and evaluated by cluster analysis(CA) and principal component analysis (PCA). Results There was no significant difference between the measured values of QAMS method and external standard method. The method of fingerprint of decoction pieces of Aurantii Fructus was established, the similarities were greater than 0.95. There were 11 common peaks in the HPLC fingerprint, compared with the control product spectrogram, peak 3 was narirutin, peak 4 was naringin, peak 5 was hesperidin, peak 6 was neohesperidin. Conclusion The QAMS method combined with fingerprint can provide a reference for evaluating the quality of decoction pieces of Aurantii Fructus.