PMP22在涎腺腺样囊性癌中的表达及临床意义

目的 观察外周髓鞘蛋白 22（PMP22）在涎腺腺样囊性癌（SACC）组织和细胞系中的表达，揭示 PMP22 与 SACC 高 转移性、神经侵犯性及预后之间的关系。 方法 收集 41 例 SACC 患者的标本，采用免疫组化 SP 法检测 PMP22 的表达。以 SACC 高 转移和非转移细胞系 SACC-LM 和 SACC-83 细胞为研究对象，利用 Western blot 检测 PMP22 的表达差异。 结果 癌旁正常组织 中 PMP22 表达明显高于 SACC 癌组织，具有统计学差异（P=0.000）；PMP22 在 T4 期 SACC 患者中表达阳性率明显低于 T1~3 期，具有统计学差异（P=0.014）；伴有神经侵犯的 SACC 患者中 PMP22 表达阳性率高于无神经侵犯者，具有统计学差异（P=0.038）；3 种组织类型的 SACC 比较，PMP22 的表达阳性率也存在统计学差异（P=0.017）；复发组表达阳性率低于不复发组（P=0.003）；不同年 龄、性别、肿瘤部位、淋巴结转移等的 SACC 患者之间 PMP22 表达阳性率均无统计学差异（P=0.632、0.790、0.458、0.692）。Western blot 显示，PMP22 在 SACC-LM 中表达高于 SACC-83（P=0.000）。 结论 PMP22 的表达与 SACC 的发生、发展及复发可能存在密 切联系，可以用于对患者预后的评估。

Expression of PMP22 in salivary gland adenoid cystic carcinoma and its clinical significance

Objective To investigate the expression of PMP22 in salivary gland adenoid cystic carcinoma (SACC) tissue and cell lines and its clinical significance. Methods The expression of PMP22 was examined with immunohistochemistry (SP method) in 41 tissue samples from patients with SACC admitted in Zhejiang Provincial Cancer Hospital from 2002 to 2013. The expression of PMP22 was detected by Western blot in metastatic SACC cell line SACC-LM and non-metastatic SACC cell line SACC-83. Results The expression of PMP22 in adjacent normal tissue was higher than that in SACC tissues (P=0.000); the expression of PMP22 in T4 SACC tissue was lower than that in T1~3 SACC tissue (P=0.014) ; the expression of PMP22 in nerve invasion group was higher than that in non-nerve invasion group (P=0.038); the expression of PMP22 in three different histological types of SACC was significantly different (P=0.017); the expression of PMP22 in recurrent group was lower than that in non-recurrent group(P=0.003). The expression of PMP22 was not correlated with age, sex, tumor locaton and lymph node metastasis (P=0.632, 0.790, 0.458, 0.692). Western blot showed that PMP22 level in SACC-LM were significantly higher than that in SACC-83 cells. Conclusion The expression of PMP22 is closely related to the occurrence and development of SACC, and it may have prognostic value for SACC.