| 体外实时监测细胞行为的新方法-压电细胞芯片(PCC) |
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| 引用本文： | 韦晓兰,莫志宏.体外实时监测细胞行为的新方法-压电细胞芯片(PCC)[J].生物医学工程学杂志,2006,23(5):1145-1148. |
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| 作者姓名： | 韦晓兰 莫志宏 |
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| 作者单位： | 1. 重庆大学,生物医学工程学院,重庆,400044
2. 重庆大学,生物医学工程学院,重庆,400044;重庆大学,化学化工学院,重庆,400044 |
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| 摘 要： | 压电细胞芯片（Piezoelectric cell-based chip，PCC）是以压电生物芯片检测技术为基础，结合体外细胞培养技术，将生活细胞作为研究对象或敏感元件，构建出的一种能实时动态监测细胞行为的多参数检测体系。该技术通过检测细胞生长过程中的△f、△Z、△D等多种参数，反映细胞生长代谢的基本功能信息。文中介绍了PCC的原理、操作方法、结果与讨论等，并对其发展方向和应用进行了展望。
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| 关 键 词： | 压电细胞芯片 实时 多参数 |
| 收稿时间： | 2004-05-26 |
| 修稿时间： | 2004-05-262004-09-05 |
A New Real-time Method Monitoring Behaviors of Living Cells In Vitro-PCC |
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| Wei Xiaolan,Mo Zhihong.A New Real-time Method Monitoring Behaviors of Living Cells In Vitro-PCC[J].Journal of Biomedical Engineering,2006,23(5):1145-1148. |
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| Authors: | Wei Xiaolan Mo Zhihong |
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| Affiliation: | College of Bimedical Engineering of Chongqing University, Chongqing 400044, China. |
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| Abstract: | A method combinating piezoelectric-biochip technique with cell culture technique in vitro has been developed.Piezoelectric cell-based chip(PCC) can continously monitor the behaviors of living cells attached to or spreaded on surfaces.The method measures the resonant frequency(f),the dissipation energy(D) and impedance(Z),in real time.These multi parameters can inflect information of cells qualitively and quantitively.This paper covers principle,materials and methods,results and discussion of the new technique,simultaniously indicates its application and trends. |
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| Keywords: | Piezoelectric cell-based chip(PCC) Real time Multi-parameter |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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