大鼠退变髓核腰交感神经干注射对交感神经干炎症因子表达的影响

目的观察X线引导下大鼠退变髓核腰交感干注射对腰交感神经干炎症因子表达的影响。方法实验用雄性SD大鼠共72只,随机分为三组:空白对照组、假手术组、髓核注射组。髓核注射组大鼠行X线下自体退变髓核悬液右侧腰交感干注射,假手术组注射等剂量生理盐水,空白对照组不做任何处理。注射后14天,取各组大鼠右侧腰交感干行组织学观察,并采用ELISA法定量分析炎性细胞因子IL-1β、IL-6和TNF-α表达变化。结果髓核注射组大鼠腰交感干组织学观察发现有明显的炎性细胞浸润及血管肿胀、充血;ELISA检测发现腰交感干IL-1β(20.1±4.40pg/ml),IL-6(26.5±8.9pg/ml)及TNF-α(60.77±6.37pg/ml)蛋白含量比空白对照组IL-1β(8.02±1.31pg/ml),IL-6(10.30±1.43pg/ml),TNF-α(24.38±4.81pg/ml)及假手术组IL-1β(7.52±2.11pg/ml),IL-6(11.58±2.56pg/ml),TNF-α(26.12±6.81pg/ml)表达显著增高(P0.05)。结论大鼠自体退变髓核导致腰交感神经干炎症因子表达升高可能与盘源性内脏痛的发生有关。

The effects of degenerative autologous nucleus pulposus injected to lumbar sympathetic trunk on expression of inflammatory cytokine in rats

Objective The aim of the study was to observe the inflammatory response of sympathetic trunk by autol-ogous degenerative nucleus pulposus （NP）injection under fluoroscopy.Methods A total 72 rats was used,which divid-ed in 3 group：nave group,sham group and NP-treated group.24 rats in NP-treated group,autologous NP suspension was injected to right sympathetic trunk.14 days after injection,right lumbar sympathetic trunk was harvested for histo-logical assessment and ELISA quantitative determination of IL-1β,IL-6,and TNF-αprotein.Results In the NP treated group,endoneural hyperemia and intensive infiltration of inflammatory cells can be seen in section of sympathetic trunk by Hematoxylin and Eosin （HE）stained,Meantime,elevated concentration of IL-1β（20.1±4.40 pg/ml）,IL-6 （26.5 ±8.9 pg/ml）and TNF-α（60.77 ± 6.37 pg/ml）by ELISA of sympathetic trunk in NP-treated group compared to naveIL-1β（8.02±1.31 pg/ml）,IL-6 （10.30±1.43 pg/ml）,TNF-α（24.38±4.81 pg/ml）and controlIL-1β（7.52± 2.11 pg/ml）,IL-6 （11.58±2.56 pg/ml）,TNF-α（26.12±6.81 pg/ml）group（P〈0.05）.Conclusion Elevated ex-pression of inflammatory cytokine,which caused by degenerative autologous nucleus pulposus inj ected to lumbar sympa-thetic trunk,may be related to discogenic visceral pain.