| 乌头碱在豚鼠和小鼠肝微粒体细胞色素氧化酶中的代谢作用研究 |
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| 引用本文： | 谢利霞,吕昌,叶玲,唐斓.乌头碱在豚鼠和小鼠肝微粒体细胞色素氧化酶中的代谢作用研究[J].中国药房,2012(7):590-593. |
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| 作者姓名： | 谢利霞 吕昌 叶玲 唐斓 |
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| 作者单位： | [1]广东医学院附属南山医院药剂科,广东深圳518052 [2]南方医科大学药学院,广州510515 |
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| 摘 要： | 目的:研究乌头碱(AC)在豚鼠和小鼠体外肝微粒体中代谢产物的差异。方法:优化2个种属肝微粒体与AC的P450反应体系,应用高效液相色谱-高分辨质谱联用(HPLC-HRMS)和超高相液相色谱-多级质谱(UPLC-MS/MS)法测定AC在2种肝微粒体中的细胞色素氧化酶(CYP)代谢产物。结果:AC在2种肝微粒体中均代谢产生8种代谢产物,其中6种为CYP代谢产物;脱甲基、脱乙基、脱氢和氧化反应是其主要代谢特征。结论:乌头碱在豚鼠和小鼠体外肝微粒体中的CYP主要代谢途径相同。
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| 关 键 词： | 乌头碱 代谢 肝微粒体 细胞色素P450氧化酶 |
Study on Metabolism of Aconitine in Liver Microsomes of Guinea Pig and Mice |
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| XIE Li-xiaDept.of Pharmacy,Nanshan Affiliated Hospital of Guangdong Medical College,Guangdong Shenzhen,China Lü Chang,YE Ling,TANG Lan.Study on Metabolism of Aconitine in Liver Microsomes of Guinea Pig and Mice[J].China Pharmacy,2012(7):590-593. |
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| Authors: | XIE Li-xiaDeptof Pharmacy Nanshan Affiliated Hospital of Guangdong Medical College Guangdong Shenzhen China Lü Chang YE Ling TANG Lan |
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| Affiliation: | (School of Pharmaceutical Sciences,Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | OBJECTIVE：To study the difference of the metabolites of aconitine（AC） in liver microsomes of guinea pig and mice.METHODS：HPLC-HRMS and UPLC-MS/MS were developed to determine the metabolites of AC in two liver microsomes,after optimizing the cytochrome P450 reaction systems of AC in two liver microsomes.RESULTS：Eight metabolites were detected in both two reaction systems,and six of them was determined as CYP-mediated metabolites.The metabolic pathways were demethylation,de-ethylation,dehydrogenation and oxidation reaction.CONCLUSION：There was no species different in the metabolic pathways of AC in liver microsomes of guinea pig and mice. |
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| Keywords: | Aconitine Metabolism Liver microsomes CYP450 |
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