| 微弧氧化钛片与大鼠牙髓干细胞的生物相容性及成骨诱导研究 |
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| 引用本文： | 黄怡,杨成,常婷,黄晓菲,刘鑫灿,吴梦娟,田元,张景辉.微弧氧化钛片与大鼠牙髓干细胞的生物相容性及成骨诱导研究[J].临床口腔医学杂志,2011,27(10):594-598. |
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| 作者姓名： | 黄怡 杨成 常婷 黄晓菲 刘鑫灿 吴梦娟 田元 张景辉 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院口腔科,湖北,武汉,430022
2. 华中科技大学同济医学院附属协和医院普通外科实验室,湖北,武汉,430022 |
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| 摘 要： | 目的：观察大鼠牙髓干细胞（dental pulp stem cells,DPSCs）在微弧氧化（micro-arc oxidation,MAO）钛片表面粘附和分化情况,初步评价MAO钛片对DPSCs的成骨诱导性能。方法：实验组：DPSCs与MAO钛片共培养,阳性对照组：DPSCs成骨诱导培养,空白对照组：DPSCs无诱导普通培养。扫描电镜（SEM）观察DPSCs在MAO钛片表面粘附情况,碱性磷酸酶（ALP）活力检测,实时定量PCR分析Runx2、Osterix、DSPP和DMP-1基因表达和钙结节茜素红染色,鉴定DPSCs的成骨能力。结果：SEM观察MAO钛片表面粗糙多孔,DPSCs在MAO钛片表面粘附紧密,有触角向微孔内伸展。ALP活力检测和钙结节染色,实验组与阳性对照组无显著差异（P〉0.05）,与空白对照组有显著差异（P〈0.05）。实验组Runx2、Osterix表达增强,DSPP和DMP-1表达无明显变化。结论：粗糙多孔的MAO钛片与DPSCs有良好的生物相容性,DPSCs在MAO钛片表面粘附紧密,具有向成骨细胞系分化的能力。
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| 关 键 词： | 牙髓干细胞 微弧氧化 成骨诱导 生物相容性 骨组织工程 |
The study of biocompatibility and osteogenesis of dental pulp stem cells on micro-arc oxidation titanium |
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| HUANG Yi,YANG Cheng,CHANG Ting,HUANG Xiao-fei,LIU Xin-can,WU Meng-juan,TIAN Yuan,ZHANG Jing-hui.The study of biocompatibility and osteogenesis of dental pulp stem cells on micro-arc oxidation titanium[J].Journal of Clinical Stomatology,2011,27(10):594-598. |
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| Authors: | HUANG Yi YANG Cheng CHANG Ting HUANG Xiao-fei LIU Xin-can WU Meng-juan TIAN Yuan ZHANG Jing-hui |
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| Affiliation: | HUANG Yi1,YANG Cheng1,CHANG Ting1,HUANG Xiao-fei1,LIU Xin-can1,WU Meng-juan1,TIAN Yuan2,ZHANG Jing-hui2.1.Department of Stomatology,2.General Surgery laboratory,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei WuHan 430022,China |
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| Abstract: | Objective:Osteogenic capacity of rat dental pulp stem cells(DPSCs) have been evaluate by observating the adhesion and differentiation ability of DPSCs on micro-arc oxidation(MAO) titanium.Method:DPSCs were challenged with MAO(experiment group),osteogenesis induction medium(positive control group) and common culture medium(blank control group) for 3 weeks,the osteogenic capacity was assessed by the assays of scanning electron microscope(SEM),biochemical tests and real time PCR.Result:SEM observation showed t... |
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| Keywords: | dental pulp stem cells micro-arc oxidation osteogenesis biocompatibility bone tissue engineering |
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