幽门螺杆菌感染诱导髓源抑制细胞在胃癌发生中的作用

目的探讨幽门螺杆菌(Hp)感染与外周血髓源抑制细胞(MDSC)水平的关联及Hp感染或非感染人群体内免疫功能的变化。方法 129例受试者分为健康组(32例)、慢性胃炎组(48例)、胃上皮内瘤变组(27例)和胃癌组(22例),快速尿素酶检测Hp。应用流式细胞术检测外周血MDSC、T细胞亚群的比例。结果 Hp感染能显著增加外周血MDSC的比例(P<0.01),而T细胞亚群的比例变化与Hp感染无关(P>0.05)。三组外周血MDSC、T细胞亚群的比例差异有统计学意义(P<0.01)。外周血MDSC比例与CD4+/CD8+比值呈显著负相关(r=-0.768),与胃癌TNM分期呈显著正相关(r=0.856)。结论 Hp感染可导致外周血MDSC比例的升高,并引起人体免疫功能紊乱,在慢性胃炎演变至胃癌的肿瘤逃逸过程中起重要作用。

Role of Helicobacter pylori infection inducing myeloid-derived suppressor cells in gastric carcinogenesis

Objective To investigate the relationship between Helicobacter pylori(Hp) infection and peripheral myeloid-derived suppressor cells(MDSC) and the changes of immune function in people with or without Hp infection.Methods A total of 129 cases was divided into 4 groups of healthy volunteers(32 cases),chronic gastritis(48 cases),gastric intraepithelial neoplasia(27 cases) and gastric cancer(22cases).The rapid urease test was performed for detecting Hp infection.The percentages of MDSC and T lymphocyte subsets in peripheral blood were detected by flow cytometry.Results The percentage of MDSC in the peripheral blood with Hp infection was obviously increased(P<0.01),which of T lymphocyte subsets was not related with Hp infection(P>0.05).The percentages of MDSC and T lymphocyte subsets were significantly different among 3 cancer groups(P<0.01).The percentage of MDSC was negatively correlated to CD4+/CD8+ ratio(r=-0.768) and positively correlated to TNM stage of gastric cancer(r=0.856).Conclusion Hp infection can lead to an increase of the percentage of MDSC in peripheral blood and abnormality of immune function,which might play an important role in the evolution of tumor immune evasion from chronic gastritis to gastric cancer.