| 银环蛇中毒死亡鉴定方法的实验研究(Ⅱ)──长碳臂活化生物素─马抗银环蛇抗体的制备 |
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| 引用本文： | 闫祖康,祝家镇.银环蛇中毒死亡鉴定方法的实验研究(Ⅱ)──长碳臂活化生物素─马抗银环蛇抗体的制备[J].法医学杂志,1995(4). |
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| 作者姓名： | 闫祖康 祝家镇 |
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| 作者单位： | 上海医科大学法医学系，中山医科大学法医学系 |
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| 摘 要： | 作者用ｄ－生物素自制Ｎ－羟基丁二酰亚胺６－生物素化酰氨已酯（长碳臂活化生物素）。质谱分子离子峰测定分子量为４５４；红外光谱分析各官能基团与分子结构式吻合。探讨了长碳臂活化生物素与精制马抗银环蛇抗体交联的最适比例。与普通活化生物素标记、辣根过氧化物酶标记的同样抗体相比，稀释滴度分别提高４—８倍。
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| 关 键 词： | N—羟基丁二酰亚胺6－生物素化酰胺已酯 生物素 蛇咬伤鉴定 |
Identification of Death Caused by Bungarotoxin: (2) Synthesis of Biotinglated Horse Anti-Bungarus IgG F (ab') 2 |
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| Yan Zulcang.Identification of Death Caused by Bungarotoxin: (2) Synthesis of Biotinglated Horse Anti-Bungarus IgG F (ab'''') 2[J].Journal of Forensic Medicine,1995(4). |
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| Authors: | Yan Zulcang |
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| Abstract: | N-hydroxysuccinimido 6-(biotinylamido) hexanoate (NHBH) prepared from d-biotin was measured with mass spectroscopy, which gave a molecular weight at 454. Further characterization by infraied spectroscopy confirmed the desired functional groups. The optimization of the molar incorporation ratios between NHBH and horse anti-Bungarus IgG (ab') 2 previously purified by affinity Chromatography is investigated. The enhancement of the dilution titer of the conjugate was 4 and 8 fold higher than that of conven tiotal biotin-theied conjugate and peroxidase conjugate, respectively. |
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| Keywords: | N-hydroxysuccinimido 6-(biotinlamido) hexanoate bungarotoxin d-biotin snake biting |
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