| 高特异性长双歧杆菌多克隆抗体的制备 |
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| 引用本文： | 徐锋,彭珍,万翠香,熊勇华,赖卫华,魏华,曾明,陈雪岚.高特异性长双歧杆菌多克隆抗体的制备[J].粉末涂料与涂装,2010,23(2). |
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| 作者姓名： | 徐锋 彭珍 万翠香 熊勇华 赖卫华 魏华 曾明 陈雪岚 |
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| 作者单位： | 徐锋,彭珍,万翠香,熊勇华,赖卫华(南昌大学食品科学与技术国家重点实验室,南昌,330047);魏华,曾明(中国药品生物制品检定所细菌一室,北京,100050);陈雪岚(江西师范大学生命科学学院,南昌,330044) |
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| 基金项目： | 国家973重点计划项目子课题，国家863计划专题项目，江西省自然科学基金 |
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| 摘 要： | 目的制备高特异性长双歧杆菌多克隆抗体,为长双歧杆菌的免疫学检测提供参考。方法以破碎的长双歧杆菌细胞碎片为抗原免疫小鼠,制备抗血清;利用偶联相同抗原的磁珠,分离纯化长双歧杆菌多抗,分别采用间接ELISA、SDS-PAGE和点杂交法检测纯化多抗的效价、纯度和交叉反应性。结果所制备的纯化多抗效价约为1∶1500,回收率约为80%,纯度可达83%。纯化的多抗有效消除了与金黄葡萄球菌的非特异性交叉反应。结论已制备出高特异性的长双歧杆菌多克隆抗体,可用于长双歧杆菌制剂的菌体检测。
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| 关 键 词： | 长双歧杆菌 多克隆抗体 磁珠 交叉反应 |
Preparation of High Specific Polyclonal Antibody against Bifidobacterium longum |
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| XU Feng,PENG Zhen,WAN Cui-xiang,et al.Preparation of High Specific Polyclonal Antibody against Bifidobacterium longum[J].Chinese Journal of Biologicals,2010,23(2). |
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| Authors: | XU Feng PENG Zhen WAN Cui-xiang |
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| Affiliation: | XU Feng,PENG Zhen,WAN Cui-xiang,et al(National Key Laboratory of Food Science , Technology,Nanchang University,Nanchang 330047,China) |
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| Abstract: | Objective To prepare the high specific polyclonal antibody against Bifidobacterium longum and provide a reference for immunological determination of Bifidobacterium longum.Methods The cell debris of Bifidobacterium longum obtained by ultrasonication was used as an antigen for immunization of mice to prepare antisera which was purified by the magnetic beads coupled with the same antigen,and determined for titer,purity and cross reactivity by ELISA,SDS-PAGE and dot hybridization respectively. Results The tite... |
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| Keywords: | Bifidobacterium longum Polyclonal antibody Magnetic beads Cross reaction |
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