| 鹿茸草中木犀草素含量测定 |
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| 引用本文： | 张舒婷,任恒鑫,吴洪斌,崔培珅.鹿茸草中木犀草素含量测定[J].黑龙江医药科学,2012,35(3):32-33. |
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| 作者姓名： | 张舒婷 任恒鑫 吴洪斌 崔培珅 |
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| 作者单位： | 佳木斯大学药学院,黑龙江佳木斯,154007 |
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| 基金项目： | 佳木斯大学大学生科技创新项目 |
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| 摘 要： | 目的：建立高效液相色谱法测定鹿茸草中木犀草素的含量方法。方法：采用Agilent XDB C18（5μm,250×4.6mm）色谱柱,以甲醇-0.1%磷酸（55：45）为流动相,检测波长350nm,流速1.0mL.min-1。结果：在25.02～150.12μg.mL-1质量浓度范围内线性关系良好,平均加样回收率为103.1%,RSD为1.27%（n=6）。结论：所建立的含量测定方法简便可行,重复性良好,可用于鹿茸草药材的质量监控。
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| 关 键 词： | 高效液相色谱 鹿茸草 木犀草素 含量测定 |
Determination of luteolin in Monochasma sheareri Maxim |
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| ZHANG Shu-ting
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REN Heng-xin
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WU Hong-bin
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CUI Pei-shen.Determination of luteolin in Monochasma sheareri Maxim[J].Heilongjiang Medicine and Pharmacy,2012,35(3):32-33. |
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| Authors: | ZHANG Shu-ting REN Heng-xin WU Hong-bin CUI Pei-shen |
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| Affiliation: | shen(College of Pharmacy of Jiamusi University,Jiamusi 154007,China) |
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| Abstract: | Objective:To develop an HPLC method for determination of luteolin in Monochasma sheareri Maxim.Methods:The samples were separated on an Agilent XDB C18(5μm,250×4.6mm) column with methanol-phosphoric acid solution(55:45) at a flow rate of 1.0mL·min-1.The wave length was set at 350 nm and the column temperature was 25℃.Results:The standard calibration curves was linear in the ranges o f 25.02～150.12μg·mL-1(r=0.999 3).The average recoveries of the method of luteolin were 103.1%(RSD=1.27%).Conclusion:The method established was successfully applied to quantify the luteolin in Monochasma sheareri Maxim collected in different places,which is helpful to control the quality. |
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| Keywords: | HPLC Monochasma sheareri Maxim luteolin assay |
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