芜菁Br14-3-3的克隆及其在非生物胁迫下的表达分析

克隆了‘津田芜菁’（Brassica rapa subsp. rapifera‘Tsuda’）通用调节因子14-3-3基因cDNA序列，命名为Br14-3-3（GenBank登录号为MK896872）。该基因全长1 113 bp，开放阅读框全长774 bp，编码含有257个氨基酸的多肽。荧光定量PCR分析Br14-3-3在‘津田芜菁’不同组织中及其在温度、脱水、渗透、ABA和无机盐等非生物胁迫下幼苗中的表达，结果表明该基因在‘津田芜菁’的花中表达量最高，幼苗中次之；低温抑制了Br14-3-3的表达，其他非生物胁迫可诱导该基因表达，暗示Br14-3-3在非生物胁迫应答中发挥功能。

Cloning and Expression Under Abiotic Stress of Br14-3-3 in Brassica rapa subsp. rapifera

In this study，we cloned the general regulatory factor 14-3-3 gene from Brassica rapa subsp. rapifera‘Tsuda’，which was named Br14-3-3 with the accession number of MK896872 in the NCBI GenBank. The full cDNA sequence of the Br14-3-3 gene was 1 113 bp，cotaining an open reading frame of 774 bp encoding a protein of 257 amino acids. We determined the expression of the Br14-3-3 gene in different tissues and under different abiotic stress conditions，such as extreme temperature，dehydration，adverse osmosis，abscisic acid（ABA）and salt stress by quantitative-PCR analysis. The results demonstrated that the highest expression levels of the Br14-3-3 gene could be reached in the flower of Brassica rapa subsp. rapifera‘Tsuda’，followed by the seedling. The expression of the Br14-3-3 gene was upregulated under aforementioned stress conditions except for low temperature，suggesting that the Br14-3-3 gene may play a role in response of abiotic stresses.