小麦白粉病抗病新基因PmHNK的遗传分析和分子标记定位

 【目的】周98165对河南省当前流行白粉菌生理小种具有较好的抗性,并且综合农艺性状优良。明确其抗白粉病基因及遗传特性,筛选与其紧密连锁的分子标记,为抗白粉病育种提供抗源和理论支撑。【方法】将周 98165与中国春杂交、自交、测交,对双亲及其杂交后代进行苗期鉴定,用小麦白粉病菌08B1进行遗传分析,利用SSR、EST-SSR技术对双亲及抗感池进行筛选和电泳分析,并结合中国春缺四体材料进行染色体定位。【结果】周98165对3个白粉菌高毒力小种抗性良好,其抗病性受1对显性核基因控制,将该基因暂命名为PmHNK。筛选了与PmHNK 连锁的5个微卫星标记,在遗传图谱上的顺序为Xbarc77、Xgwm547、Xwmc326、Xgwm299、PmHNK、Xgwm108,Xgwm299和Xgwm108分别为PmHNK两侧距离最近的标记,图距分别为4.2 cM、5.6 cM,最远标记Xbarc77与PmHNK图距为10.6 cM,并将PmHNK 定位于3BL。【结论】抗病鉴定、遗传分析结合分子标记分析结果表明,PmHNK是一个白粉病抗病新基因。

Molecular Marker Mapping and Genetic Analysis of a Novel Powdery Mildew Resistance Gene PmHNK

【Objective】 Powdery mildew is one of the most important diseases of wheat worldwide. Zhou 98165, a winter wheat provided by wheat research center of Henan Academy of Agricultural Sciences, is a novel line which has highly resistant, high yield and excellent comprehensive agricultural characteristics. Inheritance of powdery mildew resistance gene and mapping of that in Zhou 98165 with simple sequence repeat (SSR) markers were studied to formulate efficient resistance gene and strategies for breeding cultivars resistant to powdery mildew. 【Method】 F1, F2, BC1 populations were constructed on the basis of the hybridization between Zhou 98165 and a susceptible wheat variety Chinese Spring. Powdery mildew strains LY2-1, ZZ1-1 and 02B1 were used for the test the resistance and genetic analysis of those populations with the SSR and EST-SSR primers. The location of this gene on chromosome was confirmed by the materials of monosomic CS. 【Result】 Genetic analysis of Zhou 98165 indicated that the resistance to powdery mildew race 08B1 was controlled by a single dominant karyon gene, temporarily designated as PmHNK. Five SSR markers , Xbarc77, Xgwm547, Xwmc 326,Xgwm299, and Xgwm108 were linked to PmHNK as they were all located on chromosome 3BL. PmHNK, located on 3BL,closely linked to Xgwm299 and Xgwm108 with genetic distances of 4.2 cM and 5.6 cM, respectively.The farthest marker Xbarc77 was 10.6 cM to PmHNK. 【Conclusion】 Genetic analysis and resistance identification with powdery mildew isolates to Zhou98165 combined with SSR markers supports the conclusion that the highly resistance gene PmHNK is a novel gene for powdery mildew in wheat.