薯蓣丸对化疗4T1 乳腺癌小鼠 T 淋巴细胞亚群的影响

目的 评价薯蓣丸对化疗后4T1 乳腺癌小鼠免疫功能的影响，并探讨其作用机制。方法 随机 将32 只BALB/c 小鼠分为对照组、模型组、化疗组、中药组，每组8 只。除对照组外，其余组注射4T1 细胞 悬液复制荷瘤模型。成瘤后第1 天化疗组、中药组小鼠予以多西他赛治疗，1 次/7 d，共21 d ；注射多西他赛 后第2 天对中药组小鼠行中药灌胃，1 次/d，共21 d。治疗完成后处死小鼠取脾脏研磨，用流式细胞仪检测小 鼠脾脏中CD4 ＋、CD8 ＋、CD4 ＋ CD25 ＋ Foxp3 ＋调节性T 细胞（Treg）表达水平。结果 与模型组比较，化疗 组、中药组CD4 ＋、CD8 ＋、CD4 ＋ CD25 ＋ Foxp3 ＋ Treg 细胞表达水平降低，CD4 ＋ /CD8 ＋比值升高（P <0.05）； 与化疗组比较，中药组CD4 ＋细胞表达水平、CD4 ＋ /CD8 ＋比值升高，CD4 ＋ CD25 ＋ Foxp3 ＋ Treg 细胞表达水 平降低（P <0.05）。结论 薯蓣丸能改善化疗后乳腺癌小鼠免疫功能。

Effect of Dioscorea pills on T cell subgroups in mice with 4T1 breast cancer after chemotherapy

Objective To evaluate the effect of Dioscorea pills on the immunity of mice with 4T1 breast cancer after chemotherapy and its mechanism of action. Methods Thirty-two BALB/c mice were randomly divided into a control group, a model group, a chemotherapy group and a chemotherapy plus Chinese medicine group with 8 mice in each group. Except the control group, all the other groups were injected with a 4T1 breast cancer cell suspension to build a tumor-bearing mouse model. The chemotherapy group and the chemotherapy plus Chinese medicine group were treated with Docetaxel from the 1st day after tumor formation, once in 7 days, for 21 days; Dioscorea pills were given to the chemotherapy plus Chinese medicine group by gavage from the 2nd day after Docetaxel injection, once a day, for 21 days. After treatment, the mice were sacrificed and their spleens were taken out and ground, and then flow cytometry was used to detect the levels of CD4+, CD8+, CD4+CD25+Foxp3+ Treg cells. Results Compared with the model group, the levels of CD4+, CD8+ and CD4+CD25+Foxp3+ Treg cells decreased, while CD4+/CD8+ ratio increased in the chemotherapy group and the chemotherapy plus Chinese medicine group, and the differences were significant (P < 0.05). Compared with the chemotherapy group, the levels of CD4+ cells and CD4+/CD8+ ratio increased, while the level of CD4+CD25+Foxp3+ Treg cells decreased in the chemotherapy plus Chinese medicine group, and the differences were significant (P < 0.05). Conclusions Dioscorea pills can improve the immune function of mice with breast cancer after chemotherapy.