透明质酸、层粘连蛋白作为早期放射性肺损伤标志物的实验研究

目的 :探索透明质酸和层粘连蛋白对早期放射性肺损伤的诊断价值以及氟伐他汀对放射性肺损伤的治疗作用。方法 :将SD大鼠随机分为正常对照组 (C组 )、单纯照射组 (R组 )和氟伐他汀防治组 (TG组 )。TG组于照射前一周开始服氟伐他汀2 0mg·kg- 1·d- 1,直至活杀。其他组灌服等容量生理盐水。R组和TG组以直线加速器全胸部照射 ,单次剂量 2 0Gy ,于照射后5、15、3 0、60d以硫喷妥钠 40mg·kg- 1腹腔内注射。麻醉后固定 ,抽取心脏血 3ml ,分离血清。用放射免疫法测透明质酸、层粘连蛋白含量 ,统计分析用重复测量多因素方差分析和t检验。结果 :透明质酸在R组于照射后 60d较C组显著升高 (P <0 .0 1) ,TG组较R组减低 (P <0 .0 5)。层粘连蛋白在R组 3 0、60d较C组升高 (P <0 .0 5) ,TG组在相应时间减低 (P <0 .0 5)。结论 :透明质酸可作为放射性肺炎治疗情况的监测指标 ,层粘连蛋白可以作为预测纤维组织增生的一个早期指标 ;氟伐他汀对放射性肺损伤有一定的防治作用 ,用药后透明质酸、层粘连蛋白增高均受抑制

Hyaluromic acid and laminin as early markers in rats with radiation-induced lung injury

Objective: To inquire into the diagnostic value of hya luromic acid(HA) and laminin(LN) in early radiation lung injury and the effect of fluvastatin. Methods:SD female rats were randomly divided into three groups, control group(C), radiation injury group(R)and fluvastatin(Flu) treated group(T). The rats were injured with rays (20 Gy) on the chest. Prior to seven days, Flu was given to the rats in group T at a dose of 20 mg daily by gastrogavaging. The serum of the rats were collected at different times, and hyaluromic acid and laminin w ere measured by radioimmunoassay. The group data were evaluated by repeated measuring of general linear model and independen t sample t test of SPSS. Results:serum HA in R increased markedly 60 days after radiation(P<0.01), but it significantly decreased in T (P<0.05). LN in R increased on day 3 0 and 60 (P<0.05) and sign ificantly decreased in T.(P<0.05).Conclusion: HA might be an early marker of lung injury. The increased LN level in the serum might be a marker of the lung fibrosis induced by radiation injury, and fluvastatin was proved effective in preventing the inju ry.