骨质疏松动物实验中的骨物理密度检测

目的 观察骨组织物理密度的测量在骨质疏松大鼠模型判断及药物药效评价中的作用。方法 采用固体物理密度仪和DEXA分别测量大鼠的股骨和腰椎的骨密度，采用三点弯曲试验测量股骨抗弯和压缩试验测量L4的抗压等力学特性，观察骨质疏松切除卵巢模型的骨特性改变及双膦酸盐和降钙素药物的作用。结果 OVX组大鼠腰椎（L2）和股骨物理密度较正常对照组明显降低（P＜0．01，P＜0．001），L2的物理密度值与L1－5的面密度呈高度相关（r=0.934),L2和股骨物理密度变化与其最大载荷值有良好相关性（r=0.404,r=0.455)。药物对腰椎和股骨物理密度和骨生物力学等指标有不同程度提高。结论 利用固体密度仪直接测量实验大鼠骨标本的密度，可灵敏反映出各实验组之间的差异。与DEXA数据比较具有高度相关性，与骨生物力学功能的改变有良好听合。

Measurement of bone density in rat model of osteoporosis

Objective To investigate the role of bone density measurement in the assessment of rat model of osteoporosis and pharmacological effects Methods Bone density (BD) in rat right femur and lumbar vertebrae (L 4) were measured by Archimedes principle The bone mineral density (BMD) was detected by DEXA in lumbar vertebrae (L 1-5 ),The mechanical property of femur and vertebrae were evaluated by three point bending test and compression test, respectively Results The bone density was decreased in femur and L 2 of OVX rats ( P<0 01,P<0 001) and maintained or increased by elcatonin and biphosphonate Significant correlation was found between BMD and BD ( r=0 934) The BD of L 2 correlated with maximal compression load ( r=0 404) and the BD of femur correlated with maximal bending load ( r=0 455) Conclusion There is highly significant correlation between BD and BMD in rats