| 表皮生长因子受体跨膜区编码序列的克隆 |
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| 引用本文： | 马文学,余海,易平勇,金洪传,严杰.表皮生长因子受体跨膜区编码序列的克隆[J].浙江大学学报(医学版),2002,31(4):235-238. |
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| 作者姓名： | 马文学 余海 易平勇 金洪传 严杰 |
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| 作者单位： | 1. 浙江大学医学院肿瘤研究所,杭州,310009
2. 浙江大学医学院微生物学教研室,杭州,310006 |
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| 基金项目： | 浙江省自然科学基金资助项目 (399131和 30 15 80 ) |
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| 摘 要： | 目的：克隆表皮生长因子受体跨膜区（Transmembrane,TM）编码序列，为构建跨膜型超抗原和细胞因子的表达载体奠定基础。方法：以表达c-erb-B2癌基因的中国卵巢癌细胞系HO－8910细胞的RNA逆转录产物(cDNA）为模板，用两条针对c-erb-B2全长基因序列中编码TM区序列特异性的引物，采用RT－PCR方法克隆TM区片段并测序。结果：实验克隆的TM编码序列，经测序证实与Genbank中接受号为M11730的TM区序列完全一致；与接受号为X03363的TM区序列存在G→A的多态性。结论：成功地克隆了TM编码序列，并证实源于中国的卵巢癌细胞系HO－8910中所包含的表皮生长因子受体基因中编码的TM区序列存在多态性。
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| 关 键 词： | 表皮生长因子受体 跨膜区 编码 克隆 原癌基因 序列分析 基因表达 c－erb－B2 |
| 文章编号： | 1008-9292(2002)04-0235-04 |
| 修稿时间： | 2001年8月13日 |
Cloning of transmembrane domain sequence of EGFR gene |
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| MA Wen xue,YU Hai,YI Ping yong,et al.Cloning of transmembrane domain sequence of EGFR gene[J].Journal of Zhejiang University(Medical Sciences),2002,31(4):235-238. |
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| Authors: | MA Wen xue YU Hai YI Ping yong |
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| Abstract: | Objective: To clone the transmembrane(TM)domain sequence of EGFR gene and lay a good foundation for constructing the transmembrane expresssion vector of recombinant superantigens and cytokines. Methods: A pair of primers special to the sequence encoding TM domain of EGFR gene were synthesized, TM domain fragment was cloned by RT PCR, and the PCR product of TM domain sequence was ligated with the pGEM T vector and confirmed by DNA sequencing. Results: TM domain sequence was successfully cloned and verified by DNA sequencing. Conclusion: The successful cloning of TM domain sequence provides a basis for the construction of transmembrane fusion protein of Superantigen TM or Cytokines TM in cancer biotherapy. |
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| Keywords: | Proto Oncogenes Sequence analysis Gene expression Receptors epidermal growth factor Cloning molecular c erb B2 Transmembrane Domain |
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