妇科疾病中人乳头瘤病毒感染的分子流行病学调查

目的:探讨各种妇科疾病与人乳头瘤病毒感染的关系,建立不同妇科疾病和不同年龄段妇女人乳头瘤病毒感染的流行病学资料库。方法:应用反向点杂交技术分别对疾病组1 512例病人标本和正常组500例标本进行HPV检测,所得数据用SPSS 13.0软件包进行统计分析。结果:正常组500例标本中,阳性标本101例,感染率为20.2%,疾病组1 512例标本中,阳性标本523例,感染率为34.6%,其中双重感染82例,占阳性标本的15.7%,多重感染34例,占阳性标本的6.5%,而高危感染340例,感染率为22.5%,占阳性标本的65.0%,其中单纯HPV16亚型感染155例,占所有阳性标本的29.6%;不同年龄段、不同性生活史、不同妇科疾病以及不同首次性生活年龄的HPV感染检出率都有显著性差异。结论:正常组和疾病组的HPV感染率有显著性差异(2χ=36.37,P<0.01)。在HPV感染的普查中可以有重点的选择高危人群,这样更加有针对性,从而大大降低普查成本。

An epidemiological research on human papillomaviruses infections among women with gynecologic disease

Objective:To obtain the epidemiological data of Human papillomaviruses infection among women with different ages and gynecologic disease.Methods:Specimens(normal group and abnormal group)from women who wanted to do HPV detection were detected with the assay of reverse dot blot.The data was analyzed with the statistical software SPSS13.0.Results:For the normal group,20.2%(101/500) specimens were detected positive for HPV infection.1512 women were included in the abnormal group and 523(34.6%) specimens were detected positive for HPV DNA,including 407(77.8%) one-type infection,82(15.7%) diplex infection and 34(6.5%) multiple infection.Of 523 positive specimen,340(65.0%) were high-risk HPV infection,including 155(45.6%) HPV 16 type.The difference between the HPV infection of normal group and abnormal group was significant(χ2 =36.37,P<0.01).And there was significant difference of HPV infection among women with different ages,sexual history,gynecologic diseases and the ages of first sex behavior.Conclusion:In the screening of HPV infection,the high-risk population can be selected so as to decrease the cost of screening.