禁欲时间对精子参数及精浆生化指标的影响

目的 探讨禁欲时间对精子参数及精浆生化指标的影响.方法 采用精子形态检测系统下人工修正方法进行精子形态分析.采用精子质量检测系统进行精子密度、活力分析.精浆果糖含量、中性a糖苷酶、精浆锌、酸性磷酸酶等采用分光光度比色法测定.采用DTNB改进法检测精浆肉毒碱含量.前列腺特异性抗原(PSA)采用试剂盒进行检测.根据禁欲时间分为3组:G1(禁欲1～3d)组、G2(禁欲4～5d)组和G3(禁欲6d以上)组.结果G2组精子密度(70.64±63.79)×106]显著高于G1组(57.40±45.36)×106,P＜0.01],G3组精子密度(77.00±65.43)×106]显著高于G1组(57.40±45.36)×106]和G2组(70.64±63.79)×106,P＜0.01];而G3组精子活力(36.30±21.46)%]和形态正常精子百分率(17.00±9.86)%]显著低于G1组(40.47±20.60)%,(18.32±9.83)%,均P＜0.01].G3组果糖含量(20.86±15.54)μmol/1次射精]显著低于G1组和G2组(26.40±16.53)、(23.45±18.08)μmol/1次射精,P＜0.01,P＜0.05],而G3组精浆中性a-糖苷酶(47.14±33.61)mU/1次射精]、肉毒碱(28.31±21.87)mmol/L]、锌含量(2.67±1.47)mmol/L]均显著高于G1组(33.67±24.14)mU/1次射精,(20.78±16.04)mmol/L,(2.21±1.01)mmol/L]和G2组(42.05±30.63)mU/1次射精,(24.58±19.21)mmol/L,(2.07±1.01)mmol/L](P＜0.01,P＜0.05).结论 禁欲时间影响精子参数和精浆生化指标.

Effects of sexual abstinence on sperm parameters and seminal plasma biochemical indicators

Objective To study the influences of the length of sexual abstinence on sperm parameters and seminal plasma biochemical indicator.Methods Sperm concentration and motility were detected by computer assisted sperm analysis(CASA).The sperm morphology was analyzed using semiautomatic sperm morphology analyzer.Seminal plasma fructose concentration,neutral a-glycosidase,zinc,and acid phosphates concentration were measured by spectrophotometer.Improved DTNB was conducted for detection of the content of seminal plasma novain.Prostatespecific antigen(PSA)was measured with commercial kit.Three groups were defined according to the days of sexual abstinence:G1(1-3 days),G2(4-5 days)and G3(＞6 days).Results Sperm concentration of G2 was significantly higher than that of G1(P＜0.01),and of G3 than that of G1 and G2(P＜0.01).Sperm motility and per centage of morphologically normal sperm of G3 were significantly lower than that of G1(P＜0.01).Seminal plasma fructose concentration of G3 was significantly lower than that of G1 and G2(P＜0.01,P＜0.05).Seminal plasma neutral a-glycosidase,carnutine,zinc concentration of G3 was significantly higher than that of G1 and G2(P＜0.01,P＜0.05).Conclusion The length of sexual abstinence can influence sperm parameters and seminal plasma biochemical indicator.