“Methano-compost”, a booster and restoring agent for thermophilic anaerobic digestion of energy crops |
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| Affiliation: | 1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China;2. Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China;1. Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education (MOE), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China;2. College of Chemistry, Dalian University of Technology, Dalian 116024, China;3. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China;1. College of Life and Environmental Science, Guilin University of Electronic Technology, Guilin, 541004, China;2. Institute for Advanced Study, Shenzhen University, Shenzhen, Guangdong, 518052, China;3. College of Life Science, Guangxi Normal University, Guilin, 541004, China;4. College of Environment and Resource, Guangxi Normal University, Guilin, 541004, China;1. Department of Chemical Engineering, Lakehead University, 955 Oliver Road, Thunder Bay, ON, P7B 5E1, Canada;2. Faculty of Natural Resources Management, Lakehead University, 955 Oliver Road, Thunder Bay, ON, P7B 5E1, Canada;3. Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, ON, P7B 5E1, Canada;1. Department of Biosystems and Agricultural Engineering, 111 Agriculture Hall, Oklahoma State University, Stillwater, OK 74078, USA;2. Department of Microbiology and Molecular Genetics, 307 Life Sciences East, Oklahoma State University, Stillwater, OK 74078, USA;3. Prüf-und-Forschungsinstitut Pirmasens, Marie-Curie-Straβe 19, 66953 Pirmasens, Germany |
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| Abstract: | The influence of plant litter-compost of the hot rotten-phase as additional inoculum for anaerobic batch digestion of sugar beet silage (SBS) was studied. Four simultaneously driven batch-fermenters were inoculated with sewage sludge. Two of the fermenters were inoculated additionally with the same amount of organics by compost of the hot rotten-phase. Two of the fermenters were mesophilic (40 °C) and the other two were thermophilic (60 °C). The impact on the gas production rate and gas yield was observed to be boosted for thermophilic (60 °C) and only a minor effect of 6–13% for mesophilic (40 °C) digestion. The gas yield increased considerably up to 26.5% at 60 °C (batch). Also the methane content increased from 57.4% to 62.3% by adding compost (continuously run mesophilic digestion). Fluorescence In Situ Hybridization (FISH) indicated that a microbial effect was responsible for the observed stimulation of gas production rates, but not simply by increasing the bacterial counts. By analysing each fermenter for its mineral and trace element content a mineralic effect could be excluded. However, the bacterial counts by FISH of 10 different groups were somewhat ambiguous. But an effect on the presence of Chloroflexi could be demonstrated. They nearly doubled to 15–16% by supplementation with compost. Furthermore, under thermophilic conditions, the added compost induced a significant shift in the microbial composition towards hydrogenotrophic Methanobacteriales. The suggestive conclusion drawn is that this explicitly increase in hydrogenotrophic activity could alone or in combination with accompanying fermentative bacteria forces the microbial food chain towards stimulation of methane generation. |
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| Keywords: | Anaerobic digestion Biogas Methane Renewable energy Energy crops Compost |
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