SPME-GC-MS结合组学技术分析发酵椰奶特征风味与风味物质相关性

为了分析发酵椰奶特征风味与风味物质相关性,利用固相微萃取—气相色谱—质谱联用技术(Solid-Phase Micro Extraction-Gas Chromatography-Mass Spectrometry,SPME-GC-MS)分别对发酵椰奶、原味椰奶和酸牛奶中的风味成分进行测定,同时进行感官评价;并且结合组学技术从感官和风味物质方面分析了发酵椰奶的特征风味。研究结果表明,发酵椰奶感官风味特征在甜味、酸味、椰子味、奶香味、乳脂味以及醇香味感官评分较高,对发酵椰奶的喜好感主要受甜味、酸味、椰子味以及香草味的影响;而相比原味椰奶甜味过高,且伴随有较浓蒸煮味。从发酵椰奶中共检测出23种风味成分,比原味椰奶和酸牛奶分别多5种和2种;相比原味椰奶,发酵椰奶中酸类和酯类物质相对含量分别减少了11.005%和11.670%,而醇类、酮类和醛类物质相对含量分别增加了6.015%,10.295%,1.975%;酸牛奶中酸类物质是其主要风味成分,比发酵椰奶相对含量高31.79%。发酵椰奶中2,3-戊二酮、3-羟基-2-丁酮等风味成分主要贡献了果香味、奶香味和焦糖气味,酯类物质主要贡献了香草味、甜味、椰子味和蒸煮味。

Flavor components of fermented coconut milk using Omics technology combined with SPME-GC-MS

In order to analyze the correlation between characteristic flavor components and sensory flavor of fermented coconut milk. Solid-phase micro extraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) was employed to analyze the volatile flavor compounds of fermented coconut, natural coconut milk and yogurt, respectively. The sensory properties of flavor were assessed, and the characteristic flavor of the fermented coconut milk was analyzed from the sensory and flavor substances in combination with the omics technology. The results showed that the sensory flavor characteristics of fermented coconut milk were higher in sweet, sour, coconut, milky, creamy and alcoholic sensory scores; the sweetness of the original coconut milk is too high, accompanied by a thicker cooking flavor. A total of 23 flavors were detected from the fermented coconut milk, 5 and 2 of them were more than the original coconut milk and yogurt respectively. Compared with the original coconut milk, the relative content of acid and ester in the fermented coconut milk decreased by 11.005% and 11.670%, respectively; while the relative contents of alcohols, ketones and aldehydes increased by 6.015%, 10.295% and 1.975%, respectively; the acid substances in yogurt were the main flavor components, which was 31.79% higher than the fermented coconut milk. Flavored components such as 2,3-pentanedione and 3-hydroxy-2-butanone in fermented coconut milk mainly contribute to fruity aroma, milky aroma and caramel odor, and ester substances mainly contribute vanilla, sweet, coconut flavor and steamed flavor.