红树莓花色苷对D-半乳糖衰老模型小鼠的保护作用

为探究红树莓花色苷对D-半乳糖致小鼠衰老的延缓作用。通过小鼠颈背部注射D-半乳糖构建小鼠衰老模型,连续灌胃不同剂量(20、100、500 mg/(kg·d))红树莓花色苷和VC连续8周,测定小鼠胸腺、脾脏、肝脏及肾脏指数;测定血清和肝脏超氧化物歧化酶(superoxide dismutase,SOD)活性、过氧化氢酶(catalase,CAT)活性、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性及丙二醛(malondialdehyde,MDA)含量;检测皮肤SOD、CAT、GSH-Px活性和羟脯氨酸(hydroxyproline,HYP)含量;并取心脏和肾脏做苏木精-伊红染色(hematoxylin-eosinstaining,HE)病理学切片,观察多器官衰老损伤及灌胃给药后改善情况。结果显示:与模型组相比,树莓花色苷中、高剂量组胸腺和脾脏指数显著提高(p <0.05)。红树莓花色苷可以使血清、肝脏及皮肤中SOD、CAT、GSH-Px活性显著提高(p <0.05),MDA含量显著下降(p <0.05),皮肤HYP含量显著提高(p <0.05)。随着各处理组红树莓花色苷含量增加,心肌细胞逐渐接近空白组,心肌细胞排列整齐、细胞核分布均匀、出血状况缓解;肾小球数量增多,肾小囊囊腔有所减小,基底膜接近正常。说明红树莓花色苷对D-半乳糖引起的衰老小鼠有较好的保护作用。

Protective Effects of Red Raspberry Anthocyanins on D-galactose Induced Mice Aging

To investigate the anti-aging ability of red raspberry anthocyanins on D-galactose induced aging model mice.The aging model mouse was established by injecting D-galactose into the nape of the neck.These mice were given different doses continuously (low,medium,and high 20,100,500 mg/(kg·d)) of red raspberry anthocyanins and VCby gavage for 8 weeks.The indexes of thymus,spleen,liver and kidney in mice were measured and to assess the activities of superoxide dismutase (SOD),catalase (CAT),glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) content in the serum and liver,and detection of skin SOD,CAT,GSH-Px activities and hydroxyproline (HYP) content.Heart and kidney were taken for hematoxylin-eosinstaining (HE) pathological tissue sections to observe organ sensation damage and improvement of intragastric administration.The results showed that the thymus and spleen indexes in the medium and high dose raspberry anthocyanins groups were significantly higher than the model group (p < 0.05).Red raspberry anthocyanins could significantly increase SOD,CAT,GSH-Px activities in serum,liver and skin (p < 0.05) and MDA content decreased significantly (p < 0.05).Moreover,the HYP content in skin increased significantly (p < 0.05). HE pathology showed that with the increase of anthocyanin content in red raspberry in each treatment group,the cardiomyocytes gradually approached the blank group. The myocardial cells were arranged neatly and the nucleus was evenly distributed. The bleeding condition was also alleviated. The number of glomeruli increased,and the renal capsule cavity decreased.They were close to normal.In short,red raspberry anthocyanins have a good protective effect on aging mice caused by D-galactose.