铁皮枫斗多糖对小鼠急性酒精性肝损伤的保护作用

采用热水浸提方法提取铁皮枫斗多糖,研究其对小鼠急性酒精性肝损伤的保护作用。通过体积分数50%乙醇溶液灌胃诱导小鼠急性肝损伤,连续给药14 d后,摘眼球取动物血,采用血细胞计数仪测定红细胞(red blood cell,RBC)含量、红细胞比容(hematocrit,HCT)、红细胞平均体积(mean corpuscular volume,MCV)、白细胞(white blood cell,WBC)含量和血红蛋白(hemoglobin,HGB)质量浓度,采用试剂盒测定血清中谷丙转氨酶(aspartate transaminase,ALT)活力、谷草转氨酶(aspartate amino transferase,AST)活力及甘油三酯(triglyceride,TG)含量,取肝脏计算肝脏指数并制备肝匀浆,测定其中超氧化物歧化酶(superoxide dismutase,SOD)活力、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力及TG含量,并对小鼠肝脏进行组织切片观察。结果表明:与空白对照组相比,模型组血液的RBC含量、WBC含量以及血清中的TG含量、ALT活力、AST活力,肝脏指数,肝组织的TG含量、SOD活力和GSH-Px活力具有显著性差异(P<0.05或P<0.01)。相对于模型组,给药(铁皮枫斗多糖)后可显著降低血液的RBC和WBC含量,血清中的TG含量,ALT活力、AST活力,肝脏指数,肝组织的TG含量(P<0.05或P<0.01),提高肝组织的SOD和GSH-Px活力(P<0.05或P<0.01),明显改善肝组织损伤程度。铁皮枫斗多糖对酒精诱导的小鼠急性肝损伤有明显的保护作用。

Hepatoprotective Effect of Dendrobium candidum Polysaccharides on Alcohol-Induced Acute Liver Injury in Mice

The hepatoprotective effect of polysaccharides isolated from the dried stems of Dendrobium candidum by hot water extraction was evaluated on alcohol-induced acute liver injury in mice. Mice were intragastrically administrated with 50% alcohol to induce alcohol-induced acute liver injury. Thereafter, the mice were gavaged with the polysaccharides for 14 consecutive days. At the end of the experiment, blood was taken by removing eyeballs, and then the mice were sacrificed with cervical dislocation to harvest their liver tissues. Red blood cell (RBC), haematocrit, mean corpuscular volume, white blood cell (WBC) and hemoglobin were measured using a blood cell counter. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and triglyceride (TG) content in liver, as well as alanine transaminase (ALT), aspartate transaminase (AST) and TG levels in serum were measured. Histopathological liver changes were examined. The results showed that RBC and WBC levels, the serum levels of ALT, AST and TG, and SOD, GSH-Px activity and TG content in liver, and liver index significantly differed (P < 0.05 or P < 0.01) between the control and model groups. Dendrobium candidum polysaccharides significantly reduced liver index, RBC, WBC, ALT, AST and TG levels as well as enhanced the activities of SOD and GSH-Px in the liver of mice suffering from alcohol-induced acute liver injury (P < 0.05 or P < 0.01). The polysaccharides promoted recovery from hepatic tissue injury. To conclude, this study suggests that Dendrobium candidum polysaccharides have a significant hepatoprotective effect on alcohol-induced acute liver injury in mice.