水酶法提取金耳多糖的工艺优化

以云南金耳子实体为原料,拟获取酶法辅助提取金耳子实体多糖的最佳方案。以金耳多糖得率为指标,应用单因素实验结合Box-Behnken中心组合设计响应面试验,探究果胶酶及纤维素酶复合酶添加量、提取液料比、提取温度、提取时间对金耳多糖得率的影响,获得水酶法提取金耳子实体多糖最佳试验方案。结果表明,水酶法提取金耳多糖的最佳工艺条件为:复合酶添加量为20.50 mg/g,液料比为347:1 (mL/g),提取温度为52 ℃,提取时间52 min,在此条件下金耳多糖的得率为12.69%±0.52%。此法具有提取温度低、提取时间短、得率较高的优势。

Optimization of Aqueous Enzymatic Extraction of Polysaccharide from Tremella aurantialba

The fruit bodies of Tremella aurantialba in Yunnan were used as materials to obtain the optimum method of Tremella aurantialba polysaccharide aqueous enzymatic extraction. The polysaccharide extraction yield was taken as evaluation index, enzyme dosage, liquid-solid ratio, extraction temperature and extraction time were investigated through the response surface methodology design based on single factor method combined with Box-Behnken central composite method. Results showed that, the optimal parameters were determined as follows:Enzyme dosage was 20.50 mg/g, liquid-solid ratio was 347:1 (mL/g), extraction temperature was 52℃ and extraction time was 52 min. Under these conditions, the maximum extraction yield of Tremella aurantialba polysaccharide was 12.69%±0.52%. Compared with traditional method, the optimum aqueous enzymatic extraction of polysaccharide from Tremella aurantialba was obtained, which had lower extraction temperature and less extraction time but higher extraction yield.