土三七提取物对尿酸钠诱导的大鼠急性痛风性关节炎的缓解作用

为研究土三七提取物对尿酸钠诱导的大鼠急性痛风性关节炎的缓解作用,将SD大鼠随机分为正常组、模型组、土三七提取物高(300mg/kg)、中(200mg/kg)、低剂量组(100mg/kg)。各组大鼠分组给药6d后,除正常组外,其余组大鼠注射尿酸钠溶液造模。测定大鼠关节肿胀度,进行步态分析,造模48h后处死老鼠,进行苏木素-伊红(HE)染色观察关节腔积液涂片和关节滑膜浸润情况,酶联免疫吸附法测定大鼠滑膜组织中白细胞介素-1β(IL-1β)、白细胞介素-1α(IL-1α)、肿瘤坏死因子-α(TNF-α)含量。结果显示,模型组大鼠踝关节肿胀,步态跛行,滑膜组织中大量炎症细胞浸润,炎症因子IL-1β、IL-1α、TNF-α均显著(p<0.05)高于正常组,提示造模成功。土三七提取物中、高剂量组大鼠踝关节肿胀度随着时间推移均有所改善,步态肿胀也有所缓和,其中高剂量组大鼠关节肿胀抑制率一直保持在50%以上,中剂量组抑制率在致炎6h后达到47.1%,炎症因子IL-1β、IL-1α、TNF-α含量均低于模型组大鼠,且差异显著(p<0.05)。本次试验结果表明土三七提取物中、高剂量组对尿酸钠诱导的大鼠急性痛风性关节炎有积极的影响作用。

Alleviating Effect of Garcinia Extract on Acute Gouty Arthritis Induced by Sodium Uric Acid in Rats

In order to study the alleviating effect of garcinia extract on acute gouty arthritis induced by sodium urate in rats, Sprague Dawley rats were randomly divided into normal group,model group,GE group( 300,200,100 mg /kg).The rats in each group were divided into groups for six days.Except for the normal group,the rats in the other groups were given sodium urate for modeling.After the model was used to determine the swelling degree of rat joints,gait analysis was performed,and the animal was killed after eosin( HE) staining,the joint cavity effusion smear and synovial infiltration were observed,and the enzymelinked immunosorbent assay was used to determine the Leukocyte-1β( IL-1β) and interleukin-1α in synovial tissue of rats ( IL-1α),tumor necrosis factor-α( TNF-α) content.The results showed that the left ankle of the model group was swollen,the gait was limp,the inflammatory cells infiltrated in synovial tissue,and the inflammatory factor IL-1β,IL-1α,TNF-α were significantly( p < 0.05) higher than the normal group,which suggested the success of modeling.The swelling degree of ankle in the high-dose group of garcinia extract improved with time,the gait swelling was also eased,with the inhibition rate of joint swelling in the high-dose group was kept above 50% all the time,and the inhibition rate in the middle- dose group reached 47.1% after 6 h of inflammation.The inflammatory factor IL-1β,IL-1α,TNF-α content were significantly( p < 0.05) lower than these in the model group,while the low dosage group of garcinia extract had no significant difference compared with the model group.The above results showed that the high dosage group of garcinia extract have positive effects on acute gouty arthritis induced by sodium urate in rats.