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| 鼻咽癌患者血清EB病毒DNA片段的检出及临床意义 | |
| 引用本文： | 朱晓萍,陈阿英,舒莉萍,左丽,刘若英.鼻咽癌患者血清EB病毒DNA片段的检出及临床意义[J].贵阳医学院学报,2003,28(5):386-388. |
| 作者姓名： | 朱晓萍 陈阿英 舒莉萍 左丽 刘若英 |
| 作者单位： | 1. 贵阳医学院附院,儿科,贵州,贵阳,550004 2. 贵阳医学院,免疫学教研室,贵州,贵阳,550004 3. 贵阳医学院,临床微生物学和免疫学教研室,贵州,贵阳,550004 |
| 基金项目： | 贵州省教育委员会基金(1996) |
| 摘 要： | 目的：探讨鼻咽癌患者血清Epstein-Barr病毒(EBV)DNA片段检出率及其和EBV衣壳抗原IgA类抗体(VCA／IgA)的关系。方法：聚合酶链反应(PCB)加限制性内切酶酶切技术检测疱疹病毒的DNA，酶免疫组化技术检测EBV VCA／IgA抗体。结果：33例鼻咽癌患者中，抗体阳性率88％，DNA阳性率55％；对照组120例，仅1例检出EBV DNA片段。鼻咽癌患者中EBV DNA片段的检出率显著性高于对照组，但EBVDNA片段的检出率低于EBV VCA／IgA抗体的检出率。结论：进一步证实EBV与鼻咽癌有密切关系；EBV DNA片段及EBV VCA／IgA抗体检测，有利于鼻咽癌的辅助诊断。 |
| 关 键 词： | 疱疹病毒4型 人 病毒壳体 免疫球蛋白A 鼻咽肿瘤 聚合酶链反应 |
| 文章编号： | 1000-2707(2003)05-0386-03 |
| 修稿时间： | 2003年1月15日 |
An Observation on the Relationship of Serum EBV DNA Fragment and EBV VCA/IgA Antibody in Nasopharyngeal Carcinoma Patients |
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| ZHU Xiao-ping,CHEN A-ying,SHU Li-ping,ZUO Li,LIU Ruo-ying.An Observation on the Relationship of Serum EBV DNA Fragment and EBV VCA/IgA Antibody in Nasopharyngeal Carcinoma Patients[J].Journal of Guiyang Medical College,2003,28(5):386-388. | |
| Authors: | ZHU Xiao-ping CHEN A-ying SHU Li-ping ZUO Li LIU Ruo-ying |
| Abstract: | Objective: To explore the relationship of serum EBV DNA fragment and EBV VCA/IgA antibody titer in nasopharyngeal carcinoma (NPC) patients. Methods: PCR-restriction fragment length polymorphism was used to detect the EBV DNA and enzyme immunohistochemistry was used to measure the antibody titer. Results: Antibody positive rate was 88% in NPC group. DNA positive rates were 55% and 0. 83% in patients and control group respectively. Conclusions: The positive rate of EBV DNA fragment was much higher in NPC patient group than that of controls.but there was less sensitivi-ty of DNA detection than antibody detection in NPC group. |
| Keywords: | herpesvirus 4 human capsid immunoglobulin A nasopharyngeal neoplasms polymerase chain reaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
