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| 树舌多糖的分离纯化、理化性质及抗炎镇痛活性研究 | |
| 引用本文： | 焦连庆,于敏,焦莹,杨明,刘大有.树舌多糖的分离纯化、理化性质及抗炎镇痛活性研究[J].中国药师,2010,13(5):615-617. |
| 作者姓名： | 焦连庆 于敏 焦莹 杨明 刘大有 |
| 作者单位： | 1. 吉林省中医药科学院,长春,130021 2. 吉林大学 3. 长春中医药大学 |
| 基金项目： | 国家中医局重大项目，吉林省科技发展重大项目 |
| 摘 要： | 目的：分离纯化树舌多糖，获得均一多糖，并对其理化性质进行研究。对树舌多糖抗炎镇痛活性进行研究。方法：分离纯化采用DEAE离子交换色谱法和葡聚糖凝胶色谱法；纯度鉴定及分子量测定采用高效液相色谱法，示差折光检测器；单糖组成采用气相色谱质谱联用法测定。抗炎实验采用小鼠二甲苯性耳廓水肿法及大鼠角叉菜胶足肿胀法，镇痛实验采用小鼠醋酸扭体法。结果：获得三种均一多糖（GapsⅠ、GapsⅡ、GapsⅢ），峰位分子量分别为6221、5535、3518D。红外光谱证实GapsⅢ结构中有β构型异头碳，核磁共振氢谱和碳谱均证实GapsⅢ结构中有α、β构型异头碳。树舌多糖明显减轻二甲苯所致的小鼠耳廓肿胀和角叉菜致大鼠足肿胀，并减少小鼠扭体次数。结论：三种多糖分子量分布及单糖组成均不同，均主要由葡萄糖组成。 |
| 关 键 词： | 树舌多糖 分离纯化 理化性质 抗炎 镇痛 |
Study on Isolation Purification,Characterization,Antiflammatory and Antinociceptive Effects of Polysaccharides from Ganoderma Applanatum |
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| Jiao Lianqing,Yu Min,Jiao Ying,Yang Ming,Liu Dayou.Study on Isolation Purification,Characterization,Antiflammatory and Antinociceptive Effects of Polysaccharides from Ganoderma Applanatum[J].China Pharmacist,2010,13(5):615-617. | |
| Authors: | Jiao Lianqing Yu Min Jiao Ying Yang Ming Liu Dayou |
| Affiliation: | Jiao Lianqing~1,Yu Min~1,Jiao Ying~2,Yang Ming~1,Liu Dayou~3(1.Jilin Academy of Traditional Chinese Medicine Sciences,Changchun 130021,China,2.Jilin University,3.Changchun University of Traditional Chinese Medicine) |
| Abstract: | Objective:To study the characterization of homogeneous polysaccharide isolated and purified from Gaps and the activity of antiflammatory and antinociceptive of Gaps.Method:Gaps were isolated and purified by column chromatography of DEAE 52 Ionexchanged and sephadex G10;Purity and Molecular weight were determined by HPLC with RI detector;composition of monosaccharide were determined by GC/MS.Ear swelling induced by xylene in mice and Daw edema induced bv carrageenan in rats were used to observe the anti-infl... |
| Keywords: | Ganoderma applanatum Polysaccharides(Gaps) Isolation and Purification Characterization Antiflammatory effects Antinociceptive effects |
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