| 一个与净叶黄抗赤星病基因紧密连锁的SSR标记 |
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| 引用本文： | 蒋彩虹,罗成刚,任民,杨爱国,冯全福,王元英.一个与净叶黄抗赤星病基因紧密连锁的SSR标记[J].中国烟草科学,2012,33(1):19-22. |
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| 作者姓名： | 蒋彩虹 罗成刚 任民 杨爱国 冯全福 王元英 |
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| 作者单位： | 烟草行业烟草遗传育种重点实验室,中国农业科学院烟草研究所,青岛 266101 |
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| 基金项目： | 国家烟草专卖局项目“优质抗病烤烟新品种选育及育种技术研究”(110201002002) |
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| 摘 要： | 为了在分子水平上弄清烟草赤星病抗性的遗传规律,并进行遗传定位,以抗赤星病品种净叶黄和感赤星病品种NC82为亲本,构建了F1、F2、BC1 代群体。通过对该群体进行赤星病接种鉴定和抗性遗传分析,发现净叶黄对赤星病的抗性由显性多基因控制。通过分子标记群体扩增,在第M号连锁群上,筛选到一个与净叶黄的赤星病抗性基因紧密连锁的SSR标记,它与抗性基因间的遗传距离为4 cM。该标记可用于抗赤星病育种的辅助选择。
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| 关 键 词： | 烟草 净叶黄 SSR 赤星病 |
| 收稿时间： | 2011-08-11 |
A SSR Marker Tightly Linked to the Resistant Gene of Jingyehuang on Tobacco Brown Spot |
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| JIANG Caihong,LUO Chenggang,REN Min,YANG Aiguo,FENG Quanfu,WANG Yuanying.A SSR Marker Tightly Linked to the Resistant Gene of Jingyehuang on Tobacco Brown Spot[J].Chinese Tobacco Science,2012,33(1):19-22. |
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| Authors: | JIANG Caihong LUO Chenggang REN Min YANG Aiguo FENG Quanfu WANG Yuanying |
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| Affiliation: | Key Laboratory of Tobacco Genetics and Breeding, Tobacco Research Institute of CAAS, Qingdao 266101, China |
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| Abstract: | In order to study the tobacco brown spot disease resistance inheritance,and genetic mapping,F2 and BC1 populations from a cross between Tobacco Brown Spot resistant variety Jingyehuang and susceptible variety NC82 were developed.Values of disease index under artificial inoculations showed that the resistant trait of Jing-yehuang was fitted additive-dominance model.Meanwhile,a further molecular study was carried out using SSR markers based on a former genetic mapping,and a SSR marker which was tightly linked to the tobacco brown spot resistant gene located on M linkage groups with distance of 4 cM was obtained.This marker provides useful information for molecular marker assistant selection of brown spot resistance in breeding program of tobacco. |
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| Keywords: | tobacco Jingyehuang SSR Tobacco Brown Spot |
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