癫痫大鼠模型中小胶质细胞激活的脑区特点

目的 探讨小胶质细胞在癫痫中的激活特点及其在癫痫中的作用。方法 将12只Sprague-Dawley(SD)雄性大鼠随机分为对照组和癫痫组,每组各6只; 癫痫组采用氯化锂-匹鲁卡品腹腔注射的方法造模,对照组给予等量生理盐水; 癫痫发作4 d后取大鼠脑组织,采用蛋白质免疫印迹法(Western blot)和免疫组化的方法研究各组电离钙结合适配器分子-1(Ionized calcium binding adaptor molecule 1,Iba1)+小胶质细胞的表达情况。结果 与对照组大鼠模型相比,癫痫组大鼠丘脑皮层环路Iba1的表达增加,小胶质细胞的激活程度增加,且小胶质细胞特异性地在梨状皮质、丘脑室旁核、丘脑外侧背核,腹外侧部分以及腹内侧丘脑核增生。结论 癫痫发作后小胶质细胞的激活和增生存在着明显的脑区选择性,抑制丘脑皮层环路小胶质细胞的激活有望成为癫痫治疗的靶点。

Characteristics of microglial activation in epileptic rat model

Objective To investigate the activation characteristics and role of microglia in epilepsy.Methods 12 male SD rats were randomly divided into two groups equally as the control group and the epilepsy group. The epilepsy group was modeled with a lithium-pilocarpine intraperitoneal injection, and the control group was given the same amount of normal saline. Western blot and immunohistochemistry were used to study the expression of Iba1+ microglia in rats four days after an epileptic seizure.Results Compared with the control group, the expression of Iba1 in the thalamic cortical circuit and the activation degree of microglia were increased in the epileptic group, and microglia were specifically hyperplasia in the piriform cortex, paraventricular thalamic nucleus, lateral dorsal thalamic nucleus, ventrolateral part, and ventromedial thalamic nucleus.Conclusion The activation and proliferation of microglia after epileptic seizures were highly selective in brain regions. Inhibition of microglial activation in thalamic cortical circuitry was expected to target epilepsy treatment.