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Further Survey of Aflatoxin M1 in Milk Powders by ELISA
Authors:Osamu Kawamura  Dian‐Sheng Wang  Yi‐Xin Liang  Akihiro Hasegawa  Chika Saga  Angelo Visconti
Affiliation:1. Research Institute for Biosciences , Noda, Chiba 278, Japan;2. Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences , Science University of Tokyo , Ichigaya, Tokyo 162, Japan;3. Research Institute for Biosciences , Noda, Chiba 278, Japan;4. Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences , Science University of Tokyo , Ichigaya, Tokyo 162, Japan;5. Istituto Tossine e Micotossine da Parassiti Vegetali , Bari, 70125, Italy
Abstract:A survey of AFM1 residues in 58 commercial milk powder samples was carried out using an enzyme‐linked immunosorbent assay (ELISA) based on a monoclonal antibody against aflatoxin M1 (AFM1). The samples were collected from the USA (10), China (28), Italy (14), New Zealand (3) and Poland (3). The ELISA was performed without the need for clean‐up procedures. The data revealed that 4 (US), 21 (Chinese) and 1 (Polish) samples were positive for AFM1, with an average of 95.5, 102.8 and 85.0 pg g‐1 of the AFM1respectively.
Keywords:Aflatoxin M1  ELISA  milk powder
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