基于TCGA数据库挖掘揭示高表达KIF20A是肺腺癌患者预后的独立风险因素

目的:探讨KIF20A在肺腺癌中的表达状态，进一步分析KIF20A表达水平与临床病理资料和预后的关系。方法:从癌症基因组图谱（the cancer genome atlas，TCGA）下载肺腺癌RNA-seq表达和临床数据，纳入515例肺腺癌肿瘤组织样本和59例癌旁组织样本，此外，从GEO(Gene Expession Omnibus)数据库下载GSE10072数据集作为验证集，该数据集包括58例肺腺癌肿瘤组织样本和49例癌旁组织样本。分析KIF20A mRNA高低表达与临床病理特征之间的相关性通过2检验；采用Kaplan-Meier法分析临床变量与患者预后的关系；采用Cox比例风险模型探索KIF20A对肺腺癌患者预后的独立作用。结果:KIF20A 在肺腺癌组织中的表达高于癌旁组织；KIF20A mRNA表达水平与年龄、吸烟、临床分期、T分期及N分期差异显著相关，而与性别、种族、残余瘤无明显相关；高KIF20A组在总生存率和无病生存率方面较低KIF20A组差，且在校正了相关变量后，KIF20A表达仍与总生存率和无病生存率显著相关。结论:KIF20A mRNA表达水平与年龄、吸烟、临床分期、T分期及N分期显著相关；KIF20A mRNA表达水平可作为影响肺腺癌患者预后不佳的独立因素。

High KIF20A expression is an independent risk factor for prognosis in lung adenocarcinoma utilizing TCGA datasets

Objective:To investigate the effect of KIF20A expression on the clinical features and prognosis of lung adenocarcinoma.Methods:The RNA-seq expression and clinical data of lung adenocarcinoma were downloaded from the cancer genome atlas (TCGA) from 515 lung adenocarcinoma patients and 59 controls.In addition，GSE10072 dataset was download from the GEO (Gene Expession Omnibus) as a validation set，including 58 lung adenocarcinoma patients and 49 controls.The high and low expression levels were distinguished by the median value of KIF20A mRNA expression (8.44).The correlation between KIF20A mRNA expression and clinicopathological features was evaluated by Chi-Square test.Kaplan-Meier method was used to analyze the relationship between clinical variables and lung adenocarcinoma prognosis.Cox proportional hazard model was used to explore the independent effect of KIF20A on the prognosis in lung adenocarcinoma.Results:The expression level of KIF20A in lung adenocarcinoma was higher than controls.The expression level of KIF20A mRNA was closely significantly related to age，tobacco smoking history，clinical stage，T and N stage(P<0.05)，and no significant correlation with gender，race，residual tumor (P>0.05).Patients with high KIF20A expression exhibited poor prognosis compared with patients with low KIF20A expression.After correcting the covariates，KIF20A expression remained significantly associated with overall survival and disease-free survival (P<0.05).Conclusion:The expression level of KIF20A mRNA was significantly correlated with age，tobacco smoking history，clinical stage，T and N stage in patients with lung adenocarcinoma.KIF20A mRNA expression can be an independent risk factor for prognosis in lung adenocarcinoma patients.