炎性细胞因子在膝骨关节炎中的表达及与高敏 C反应蛋白和红细胞沉降率的相关性

目的 探讨膝骨关节炎（KOA）患者血清和关节液中炎性细胞因子白细胞介素（IL）-1β、肿瘤坏死因子 （TNF）-α、IL-6 的表达特点及其与高敏 C 反应蛋白（hs-CRP）和红细胞沉降率（ESR）的相关性。方法 收集 85 例 KOA 患者和 80 例健康对照者（HC）的外周血标本，收集 25 例 KOA 患者的关节液标本。采用酶联免疫吸附试验 （ELISA）检测血清和关节液中IL-1β、TNF-α、IL-6水平，采用胶乳免疫比浊法检测血清hs-CRP水平，采用全自动动 态血沉仪检测ESR。Pearson相关分析KOA患者血清和关节液IL-1β、TNF-α、IL-6与血清hs-CRP及ESR的相关性。 结果 KOA患者各组血清IL-1β、TNF-α、IL-6与健康对照组相比表达水平均升高（均P＜0.01），且其表达水平随着 病情严重程度的增加而升高；KOA患者关节液中IL-1β、TNF-α、IL-6表达水平高于血清（均P＜0.05）；KOA患者各组 血清 hs-CRP、ESR 均高于 HC 组（均 P＜0.01）；KOA 患者血清 IL-1β、TNF-α、IL-6 水平与 hs-CRP（r 分别为 0.489、 0.426、0.389）、ESR（r分别为0.348、0.423、0.394）均呈正相关（均P＜0.05）；KOA患者关节液IL-1β、TNF-α、IL-6表达 水平与血清 hs-CRP（r 分别为 0.547、0.644、0.511）、ESR（r 分别为 0.564、0.579、0.589）均呈正相关（均 P＜0.01）。结 论 炎性细胞因子IL-1β、TNF-α、IL-6与hs-CRP、ESR检测可能成为辅助KOA早期诊断、病情及预后评估的潜在血 清学标志物。

The expression of inflammatory cytokines and its relationship with high sensitivity C-reactive protein and erythrocyte edimentation rate in knee osteoarthritis

Objective To investigate the expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and IL-6 and their correlation with high sensitivity C-reactive protein (hs-CRP) and erythrocyte sedimentation rate (ESR) in patients with knee osteoarthritis (KOA). Methods Serum samples were obtained from 85 patients with KOA and 80 healthy controls (HC). Joint fluid samples were obtained from 25 patients with KOA. The levels of IL-1β, TNF-α and IL-6 in serum and joint fluid were measured by enzyme-linked immnuosorbent assay (ELISA). The serum levels of hs-CRP were measured by Latex immunoturbidimetry assay. Serum levels of ESR were measured by automatic ESR monitor. The correlations between IL-1β, TNF-α, IL-6, hs-CRP and ESR were evaluated using Pearson correlation test. Results Serum levels of IL-1β, TNF-α and IL-6 were significantly higher in KOA group compared with those of HC group (all P＜0.01). Moreover, the levels of IL-1β, TNF-α and IL-6 increased with the increase of clinical severity. The levels of IL-1β, TNF-α and IL-6 were significantly higher in joint fluid than those in serum (all P＜0.05). The levels of hs-CRP and ESR were higher in KOA group than those of HC group (all P＜0.01). The serum levels of IL-1β, TNF-α and IL-6 were positively correlated with hsCRP (r=0.489, 0.426 and 0.389, respectively) and ESR (r=0.348, 0.423 and 0.394, respectively). The levels of IL-1β, TNF- α and IL-6 in joint fluid were positively correlated with serum levels of hs-CRP (r=0.547, 0.644 and 0.511, respectively) and ESR (r=0.564, 0.579 and 0.589, respectively). Conclusion Our data show that the detection of inflammatory cytokines and hs-CRP, ESR can be used as potential biomarkers for early diagnosis, disease evaluation and prognosis assessment of KOA.