Dissection of genetic effects of quantitative trait loci (QTL) in transgenic cotton |
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Authors: | Yongshan Zhang Shuxun Yu Xiangmo Guo Zhiwei Wang Qinglian Wang Li Chu |
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Affiliation: | (1) College of Plant Science and Technology, Huazhong Agriculture University, Wuhan, Hubei, 430070, China;(2) Cotton Research Institute, CAAS, Anyang, Henan, 455000, China;(3) College of Life Science, Henan Normal University, XinXiang, Henan, 453007, China |
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Abstract: | When alien DNA inserts into the cotton genome in a multicopy manner, several quantitative trait loci (QTLs) in the cotton
genome are disrupted; these are called dQTL in this study. A transgenic mutant line is near-isogenic to its recipient, which
is divergent for the dQTL from the remaining QTLs. Therefore, a set of data from a transgenic QTL line mutated by Agrobacterium-mediated transformation (30074), its recipient and their F1 hybrids, and three elite lines were analyzed under a modified
additive-dominance model with genotype × environment interactions in three different environments to separate the genetic
effects due to dQTL from whole-genome effects. Our result showed that dQTL had significant additive effects on lint percentage,
boll weight, and boll number per square meter, while it had little genetic association with fiber traits, seed cotton yield,
and lint yield. The dQTL in 30074 significantly increased lint percentage and boll number, while significantly decreasing
boll weight, having little effect on fibre traits, while those from the recipient and three elite lines showed significant
genetic effects on lint percentage. In addition, the remaining QTL other than dQTL had significant additive effects on seed
cotton yield, fruiting branch number, uniformity index, micronaire, and short fibre index, and significant dominance effects
on seed cotton yield, lint yield, and boll number per square meter. The additive and dominance effects under homozygous and
heterozygous conditions for each line are also predicted in this study. |
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Keywords: | Quantitative trait loci (QTL) Genetic effects Modified additive-dominance model QTL mutant |
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