牛卵母细胞体外成熟培养研究进展

作为胚胎生物工程技术研究的基础之一的牛卵母细胞体外成熟培养技术自上世纪30年代起至今,许多科学研究人员在牛卵母细胞体外生长、发育领域做了大量工作,对牛卵母细胞培养条件进行了广泛而深入地研究,并取得了较大的进展,目前牛卵母细胞体外成熟培养技术体系已建立,但在卵母细胞成熟机理、成熟调控方面尚存在许多尚未阐明的问题和一些亟待解决的问题。本文从卵母细胞的来源、卵母细胞体外培养条件、培养液的完善及卵母细胞成熟判定方法等方面详尽介绍了当前牛卵母细胞体外培养技术的研究进展。

Research Advances on in Vitro Maturation of Bovine Oocyte

As one of the basis in embryo biotechnology and engineering research, the in vitro maturation of bovine oocyte had been extensively investigated since 1930's, the great progress had been achieved on in vitro growth, development, and cultural conditions of bovine oocytes. At present, although the in vitro maturation and culturing system of bovine oocyte had been established, the maturation mechanism of oocyte and maturation regulation remained unclear. The research advances of in vitro maturation technology in bovine oocyte were reviewed from the origin of bovine oocyte, the in vitro maturation condition, the improvement of culturing buffer and the maturation evaluation etc.