| 双乙酰-肌酸-CTMAB体系共振光散射法测定尿中痕量α-萘酚 |
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| 引用本文： | 袁康秀,袁玉坤,史林飞,周斌,王永生.双乙酰-肌酸-CTMAB体系共振光散射法测定尿中痕量α-萘酚[J].应用化工,2011,40(6). |
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| 作者姓名： | 袁康秀 袁玉坤 史林飞 周斌 王永生 |
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| 作者单位： | 南华大学公共卫生学院,湖南衡阳,421001 |
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| 基金项目： | 湖南省大学生研究性学习和创新性实验计划项目 |
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| 摘 要： | 在弱碱性介质中,α-萘酚被双乙酰氧化为α-萘醌,α-萘醌与肌酸生成荧光化合物,该化合物与十六烷基三甲基溴化铵生成离子缔合物,使体系的共振光散射增强,在1.44×10-9~2.00×10-7mol/L范围内,共振光散射强度(RLS)与α-萘酚浓度呈良好线性关系,回归方程为:△I=186.9c(×10-8mol/L)+84.89,r=0.999 1,检出限为4.31×10-10mol/L,样品的加标回收率为96.0%~104.0%,RSD=0.80%~3.42%,该方法可用于尿液中痕量萘酚的测定。
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| 关 键 词： | α-萘酚 肌酸 双乙酰 十六烷基三甲基溴化铵 共振光散射 |
Determination of trace α-naphthol in urine based on diacetyl creatine-CTMAB system by resonance light scattering method |
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| YUAN Kang-xiu,YUAN Yu-kun,SHI Lin-fei,ZHOU Bin,WANG Yong-sheng.Determination of trace α-naphthol in urine based on diacetyl creatine-CTMAB system by resonance light scattering method[J].Applied chemical industry,2011,40(6). |
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| Authors: | YUAN Kang-xiu YUAN Yu-kun SHI Lin-fei ZHOU Bin WANG Yong-sheng |
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| Affiliation: | YUAN Kang-xiu,YUAN Yu-kun,SHI Lin-fei,ZHOU Bin,WANG Yong-sheng(College of Public Health,University of South China,Hengyang 421001,China) |
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| Abstract: | A new resonance light scattering(RLS) method is used for the determination of α-naphthol in urine.In weak alkali medium,α-NAP was oxidized by diacetyl to form α-naphthoquinone,which formed a fluorescence compound with creatine.Then the fluorescence compound reactsed with CTMAB to form ionic complexes.The intensity of RLS at λmax 580 nm was in proportion of α-NAP concentration in the range of 1.44×10-9~2.00×10-7 mol/L.The linear regression equation was ΔI = 186.9c(×10-8 mol/L) + 84.89 with a correlation coefficient of 0.999 1,and the limit of detection was 4.31×10-10 mol/L.The method had been applied for the determination of α-NAP in urine.The recoveries were in the range of 96.0%~104.0%,and the RSD were 0.80%~3.42%. |
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| Keywords: | α-naphthol creatine diacetyl cetyltrimethylammonium bromide resonance light scattering |
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