核素^147Pm在细胞内滞留的电镜放射自显影研究

木文用电镜放射自显影术研究了重核裂片~(147)Pm在体内亚细胞水平滞留动态。发现~(147)Pm的内污染沉积早期在血细胞和血管内皮细胞中,尤以在红细胞的沉积为多。~(147)Pm呈选择性滞留于肝细胞亚微结构中,可较多的掺入枯否细胞、肝细胞核和核仁内,在胞质中的糙面内质网、线粒体、微体和糖元中亦有滞留,且主要沉积于糙面内质网和线粒体,在高尔基复合体与糙面内质网所芽生的转移小泡融合处,亦有~(147)Pm滞留的放射自显影象。而在肾小体足细胞的线粒体和溶酶体中,有浓集的放射自显影径迹呈现。在肾近端小管曲部上皮细胞核中和胞质的线粒体和微体中,亦有较多的~(147)Pm滞留。 随着观察时间的延续,~(147)Pm在骨有机质亚微结构中呈现选择性、持续性的沉积增多。可浓集于破骨细胞和成骨细胞核中。此外在骨组织细胞间质中的胶原纤维部位,亦有较多的~(147)Pm沉积,难以排除。

ACCUMULATION OF RADIONUCLIDE ~(147)Pm IN SUBCELLULAR LEVEL BY ELECTRON MICROSCOPIC AUTORADIOGRAPHY

This study investigated the subcellular localization of radionuclide ~(147)Pm in tissue cells by electron microscopic autoradiography. The early risk of inter- nal contaminated accumulation of ~(147)Pm was in blood cells and endothelial cells, espe- cially in red blood cells where more numerous of autoradiographic tracks appeared. Then ~(147)Pm was selectively deposited in ultrastructure of liver cells, such as in nucleus, nucleous, rough endoplasmic reticulum, mitochondria and microbodies. In organelles ~(147)Pm was mainly accumulated in rough endoplasmic reticulum and mitochondria. Au- toradiographic tracks especially localized in combined point between Golgi complex and transitive vesicle of rough endoplasmic reticulum. Autoradiographic study also demon- strated, where dense tracks appeared in mitochondria and lysosome of podal cells within renal corpuscle. In nucleus as well as in mitochondria and microbodies within epicyte of kidney near-convoluted tubule where also more numerous tracks of ~(147)Pm accumula- ted. With the protongation of observing time, ~(147)Pm was selectively and steadily depo- sited in subcellular level of organic component bone. Electron microscopic autoradiogra- phy showed, that substential amounts of ~(147)Pm was taken up into the nuclear fraction of osteoclasts and osteoblasts. In addition, where more numerous tracks of ~(147)Pm was deposited in collagenous fibre within interstitial of bone cells and was hardly excreted.