| 非小细胞肺癌胸水细胞块ALK融合基因检测分析 |
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| 引用本文： | 朱礼阳,徐建平,于忠和,赵洁婷,叶伟,朱东波,孙晓,宋蓉蓉,许春伟.非小细胞肺癌胸水细胞块ALK融合基因检测分析[J].国际病理科学与临床杂志,2016(12):1961-1964. |
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| 作者姓名： | 朱礼阳 徐建平 于忠和 赵洁婷 叶伟 朱东波 孙晓 宋蓉蓉 许春伟 |
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| 作者单位： | 1. 陆军总医院肿瘤科,北京,100700;2. 安徽省胸科医院病理科,合肥,230032;3. 福建省肿瘤医院病理科,福州,350014 |
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| 基金项目： | 国家自然科学基金(81372489)，北京市科技计划课题(2131100006813032)。This work was supported by the National Natural Science Foundation of China(81372489)，Beijing Municipal Science and Technology Plan Project(2131100006813032)，P. R. China |
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| 摘 要： | 目的：探讨非小细胞肺癌(non-small cell lung cancer，NSCLC)胸水细胞块在间变性淋巴瘤激酶(anaplastic lymphoma kinase，ALK)融合基因检测中的临床价值。方法：采用突变扩增系统PCR(ARMS-PCR)法检测215例NSCLC细胞块和404例NSCLC组织块中ALK基因的三类融合类型，并检测细胞块同时送检组织块的患者74例的一致性。结果：细胞块ALK基因融合阳性26例，阳性率12.09%(26/215)；组织块ALK基因融合阳性25例，阳性率6.19%(25/404)；74例有组织块对照的细胞块ALK融合基因结果一致性有67例，一致率达90.54%(67/74)，其中细胞块ALK融合基因的阳性率14.86%(11/74)，组织块阳性率18.92%(14/74)。结论：NSCLC胸水细胞块ALK融合基因的阳性率略高于组织块；有恶性胸水的NSCLC患者原发灶组织发生ALK融合基因阳性的概率较高。
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| 关 键 词： | 肺肿瘤 非小细胞肺癌 细胞块 间变性淋巴瘤激酶 |
Analysis of cell blocksALK fusion gene in pleural effusion of non-small cell lung cancer |
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| Abstract: | Objective: To investigate the clinical value of the cell blacks for anaplastic lymphoma kinase (ALK) fusion gene detection in non-small cell lung cancer (NSCLC).Methods: 215 cases of cell block from pleural effusion of non-small cell lung cancer were collected. hTree types ofALK fusion and 404 cases of tissue block were detected by ARMS-PCR method. hTe consistency ofALK fusion was examined in 74 cases of patients with tissue block and cell block.Results:ALK fusion was found in 26 of 215 cell blocks, the positive detection rate was 12.09%.ALK fusion was detected in 25 of 404 tissue blocks, the positive detection rate was 6.19%. 67 cases in the 74 (90.54%) cases had the same result as tissue block.ALK fusion was detected in 11 of 74 (14.86%) cell blocks, and 14 of 74 (18.92%) tissue blocks.Conclusion: hTe rate ofALK fusion in cell blocks of non-small cell lung cancer is higher than that in matched tissue blocks. hTe patients with malignant pleural effusion are likely to tend toALK fusion. |
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| Keywords: | lung neoplasm non-small cell lung cancer cell block ALK |
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