毛细管电泳法同时测定元胡止痛片中欧前胡素和异欧前胡素的含量

目的:建立以毛细管电泳法同时测定元胡止痛片中欧前胡素和异欧前胡素含量的方法。方法:以盐酸小檗碱为内标,采用未涂层弹性融硅石英毛细管柱(55cm×75μmID,有效长度47cm),以20mmol·L-1磷酸二氢钠+100mmol·L-1十二烷基磺酸钠+80%甲酰胺溶液(pH7.12)为运行缓冲液,分离电压为15kV,重力进样5s(高度15cm),检测波长为254nm。结果:欧前胡素、异欧前胡素检测浓度分别在8.0～40.0μg·mL-(1r=0.9987)、4.0～20.0μg·mL-(1r=0.9990)范围内与各自峰面积积分值呈良好线性关系;二者平均回收率分别为101.3%和100.2%,RSD分别为3.24%和1.44%(n=6)。结论:本法简便、快速、准确,可用于元胡止痛片的质量控制。

Simultaneous Determination of Imperatorin and Isoimperatorin in Yuanhu Zhitong Tablet by Capillary Electrophoresis

OBJECTIVE：To establish capillary electrophoresis method for the determination of imperatorin and isoimperatorin in Yuanhu zhitong tablet. METHODS：The separation was performed on a uncoated fused silica capillary （55 cm×75 μm ID,effective length of 47 cm） with 20 mmol·L-1 NaH2PO4＋100 mmol·L-1 SDS＋80%formamide as buffer solution （pH=7.12）. The separation voltage was 15 kV and the sample was injected by gravity （5 s,15 cm）. The detection wavelength was 254 nm. RESULTS：The linear range of imperatorin and isoimperatorin were 8.0～40.0 μg·mL-1（r=0.998 7） and 4.0～20.0 μg·mL-1（r=0.999 0）. The average recovery was 101.3% for imperatorin （RSD=3.24%,n=6） and 100.2% for isoimperatorin（RSD=1.44%,n=6）. CONCLUSION：The method is convenient,rapid and accurate for the quality control of Yuanhu zhitong tablet.