| 治疗用乙型脑炎单克隆抗体注射液的研制 |
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| 引用本文： | 过琴媛,张碧如,邹林,寿伟华,李鸿斌,全昱东,万邦礼,李玲玲,黄文萱,王兵,宋爱京,马文煜,黄庆生.治疗用乙型脑炎单克隆抗体注射液的研制[J].粉末涂料与涂装,1997,10(1):16-20. |
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| 作者姓名： | 过琴媛 张碧如 邹林 寿伟华 李鸿斌 全昱东 万邦礼 李玲玲 黄文萱 王兵 宋爱京 马文煜 黄庆生 |
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| 作者单位： | 卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100 |
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| 基金项目： | 国家“八五”重点攻关项目!85-722-18-01 |
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| 摘 要： | 用清洁级F1代小鼠制备日本脑炎病毒单克隆抗体腹水，经辛酸沉淀、硫酸铵盐析粗提后，再通过凝胶过滤柱层析纯化。每毫升腹水可得到单克隆抗体4～4．5mg，纯度大于95％，回收率为60％～70％，蚀斑中和法测定活性大于5×105，无鼠源病毒污染，经各项检测均符合人用鼠源性单克隆抗体的要求。按有关规程研制成治疗用乙型脑炎单克隆抗体注射液，经全面检定符合临床试验的标准。
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| 关 键 词： | 抗乙型脑炎病毒单抗 凝胶过滤 人用鼠源性单抗 |
Preparation of Therapeutic Japanese Encephalitis McAb |
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| Guo Qinyuan, Zhang Biru, Zou Lin et al.Preparation of Therapeutic Japanese Encephalitis McAb[J].Chinese Journal of Biologicals,1997,10(1):16-20. |
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| Authors: | Guo Qinyuan Zhang Biru Zou Lin |
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| Abstract: | The McAb to Japanese encephalitis were extracted from the ascitic fluid from clean F1 mice by octanic acid and (NH4)2SO4 precipitations, then purified by gel filtration column chromatography. 4-4. 5mg McAb was extracted from 1ml asctic fluid. The McAb was not contaminated by mouse-derived viruses, and the activities detected by plaque neutralizeation test were above 5 ×105. The purity and recovery rate were over 95 % and 60% ~70%respectively. All the detection results of the other items met the requirements of mouse-derived McAbs for human use. Therapeutic injections were prepared with the McAbs according to the related requirements. Overall detection results proved that the injections could be used for clinical trials. |
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| Keywords: | JE McAb Gel filtration Mouse-derived McAbs for human use |
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