抑癌扶正平衡Ⅱ号方对乳腺癌小鼠移植瘤生长转移的实验研究

目的]观察抑癌扶正平衡Ⅱ号方对乳腺癌小鼠移植瘤生长及肺转移的抑制作用，并探索其抗肿瘤效应与硫酸乙酰肝素蛋白聚糖的相关性。方法]建立4T1乳腺癌小鼠模型，随机分为空白组、模型组、低分子肝素组、抑癌扶正平衡Ⅱ号方高、中、低剂量组，连续给药14 d后处死取材。测定移植瘤瘤质量和计数肺转移结节个数，并计算瘤质量抑制率及肺转移抑制率。ELISA法检测各组小鼠移植瘤、转移瘤、血清中硫酸乙酰肝素蛋白聚糖（HSPG）的表达。结果]中药各组瘤质量、肺转移结节数随中药剂量增高而下降（P<0.05）；高剂量中药组瘤质量抑制率及肺转移抑制率均优于低分子肝素组（P<0.05）。中药各组移植瘤、转移瘤、血清中HSPG的表达随中药剂量增高而上升（P<0.05）；高剂量中药组移植瘤、转移瘤、血清中HSPG的表达高于低分子肝素组（P<0.05）。结论]抑癌扶正平衡Ⅱ号方可显著抑制乳腺癌小鼠移植瘤的增殖和肺转移，其作用机制可能与增高硫酸乙酰肝素蛋白聚糖的表达有关。

Systematic review of Fufang Xueshuan Tong combined with calcium dobesilate in treating diabetic retinopaphy

Objective] To evaluate the therapeutic effect of Fufang Xueshuan Tong combined with calciumdobesilate in treating diabetic retinopaphy. To design a systematic review of randomized controlled trials.Methods] Search databases as CNKI,VIP,Wanfang,CBM,PubMed and Cochrane Library (time from build libraries to March 2018) relevant journals and the literature of RCTs of the combination of Fufang Xueshuan Tong and calciumdobesilate in treating diabetic retinopaphy were all enrolled. To evaluate the bias of the literature according to the standard of the Cochrane system evaluation member manual bias risk assessment and extract the data. The meta-analyses were performed with Review Manager 5.3 software.Results] Thirteen articles were enrolled. The result of Meta-analyses showed that intervention measures could improve the clinical comprehensive effectOR=5.69,95%CI(3.54,9.14),P<0.000 01],improve fundus situation(vision grayscale valuesWMD=-1.00,95%CI(-1.15,-0.86),P<0.000 01],the volume of hemangiomaWMD=-3.43,95%CI(-4.22,-2.64),P<0.000 01],the contact areaWMD=-1.06,95%CI(-1.39,-0.72),P<0.000 01]),increase TCM symptom complex scoreWMD=-3.93,95%CI(-4.87,-2.99),P<0.000 01], reduce the whole blood viscosityWMD=-0.61,95%CI(-1.01,-0.22),P<0.002],reduce recurrenceOR=0.13,95%CI(0.04,0.47),P=0.002]. Besides,four articles indicated that fufangxueshuantong combined with calciumdobesilate could reduce the levels of VEGF,IGF-1 and hs-CRP.Conclusion] The combination of Fufang Xueshuan Tong and calciumdobesilate would enhance the comprehensive therapeutic effect of diabetic retinopaphy. However,we need more high-quality RCTs to improve the research.