小鼠卵母细胞玻璃化冷冻的研究

目的　对小鼠卵母细胞玻璃化的方法和冷冻液进行研究。方法　用两种玻璃化冷冻液EFS4 0 (高钠 )、DEFS4 0 (低钠 )对ICR、C57BL 6J小鼠卵母细胞进行冷冻 ,0 5mol L蔗糖液解冻 ,体外授精前对部分解冻后的C57BL 6J小鼠卵母细胞进行透明带切割。结果　DEFS4 0冷冻液冷冻效果明显高于EFS4 0冷冻液 ,ICR小鼠卵母细胞解冻后成活率达 75 2 %、体外授精率 (2 4 6 % )与对照组相比差异无显著性 ,C57BL 6J小鼠卵母细胞解冻后成活率为 87 1%、卵母细胞切割后体外受精率 (36 0 % )与对照组相比差异也无显著性。结论　冷冻液中钠离子浓度影响小鼠卵母细胞冷冻效果 ,用DEFS4 0 (低钠 )冷冻液进行玻璃化冷冻可以取得理想的冷冻效果。

Study on Vitrifaction of Mouse Oocytes

Objective To explore vitrified method and cryopreservative solution of mouse oocytes. Method The oocytes of ICR and C 57 BL/6J mice were vitrified with EFS40(high concentration of sodium) and DEFS40(low concentration of sodium),thawed with 0.5mol/L sugar solution.Before fertilization in vitro, zona-pellucieda of C 57 BL/6J mice thawed oocytes were dissected. Results Considering the vitrified effect, DEFS40 is better than EFS40. The oocyte survival rate of ICR mice reaches 75.2% after thawed. The fertilization rate reaches 24.6% and the difference is not remarkable compared to the control.And the oocyte survival rate of C 57 BL/6J mice reaches 87.1% after thawed. The oocyte fertilized rate which zona-pellucieda were dissected reaches 46.0% and the difference is not remarkable compared to the control.Conclusion The vitrified effect of mice oocyte is influenced by the concentration of sodium.The perfect effect could be obtained when the oocytes were vitrified with DEFS40.