| 重组人血管生长素的鉴定 |
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| 引用本文： | 张宏斌,江悦华,王捷,武婕,杨太成,吴锦银.重组人血管生长素的鉴定[J].中国免疫学杂志,2001,17(11):613-614,617. |
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| 作者姓名： | 张宏斌 江悦华 王捷 武婕 杨太成 吴锦银 |
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| 作者单位： | 广州军区广州总医院医学实验科 |
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| 基金项目： | 本课题受广东省自然科学基金(960668)和军队自然科学基金(96D0331)资助 |
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| 摘 要： | 目的：为了鉴定自行制备的重组人血管生长素。方法：采用凝胶扫描法分析纯度；ELISA等方法检测特异性；鸡胚绒毛尿囊膜实验检测生物活性，并与商品化标准品进行免疫结合性的对比研究。结果：重组血管生长素纯度达96%，具有促进血管生长的生物活性；而且较标准品有理想的免疫结合性。结论：该重组人血管生长素可进一步的实验和临床应用研究。
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| 关 键 词： | 重组人血管生长素 生物学活性 鉴定 rhANG |
| 文章编号： | 1000-484(2001)11-0613-03 |
The identification of recombinant human angiogenin |
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| ZHANG Hong Bin,JIANG Yue Hua,WANG Jie et al.The identification of recombinant human angiogenin[J].Chinese Journal of Immunology,2001,17(11):613-614,617. |
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| Authors: | ZHANG Hong Bin JIANG Yue Hua WANG Jie |
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| Affiliation: | ZHANG Hong Bin,JIANG Yue Hua,WANG Jie et al Department of Medical Research,Guangzhou Army General Hospital,Guangzhou 510010 |
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| Abstract: | Objective:To detect the biological activity of the recombinant human angiogenin Methods:Gel scanning was used to analyze the purity of the prepared rhANG ELISA and chicken CAM assay were used to detect specificity and biological activity The prepared rhANG was compared with the standard rhANG Results:The purity of the prepared rhANG is 96% and it could induce the angiogenesis The immunity of the prepared rhANG is better than that of the standard Conclusion:The prepared rhANG could be used for the further basic and clinical research |
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| Keywords: | rhANG Biological activity Identification |
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