一氧化氮诱导肝癌细胞凋亡过程bcl-2和bax mRNA表达水平的变化

目的 探讨在一氧化氮(NO)诱导肝癌细胞凋亡过程bcl-2和bax mRNA表达水平的动态变化。方法 用NO供体硝普钠(SNP)诱导SMMC-7721和HepG2肝癌细胞株凋亡，RT-PCR的方法检测bcl-2、ba xmRNA表达水平，并采用凝胶分析系统进行半定量分析。结果 1．0mmol/L可降低SMMC-7721和HepG2细胞bcL2、bax mRNA的表达水平，提高bax/bc1-2 mRNA的比值并呈时间依赖性。HepG2细胞bcl-2和bax mRNA降低的幅度较SMMC-7721细胞小(P＜0．05)，开始变化的时间也较SMMC-7721细胞迟。结论 NO诱导肝癌细胞株的凋亡，可能与其bcl-2、bax mRNA表达水平变化导致bax/bc1-2 mRNA比值上升有关。

Kinetics of bcl-2 and bax mRNA Expression in Nitric Oxide-induced Apoptosis of Human Hepatocellular Carcinoma Cells Lines in Vitro

Objective To investigate the kinetics of bcl\|2 and bax mRNA expression in nitric oxide induced apoptosis of human hepatocellular carcinoma cells. Methods Sodium nitroprusside(SNP) was used to induce apoptosis in human hepatocellular carcinoma cell lines. The expression of bcl\|2 and bax mRNA in the cell was detected by RT\|PCR. The PCR products were separated on gel and densitometric analysis of bands allow semi\|quantification. Results The levels of bcl\|2 and bax mRNA expression were reduced and the ratio of bax/bcl\|2 mRNA was elevated in both SMMC\|7721 and HepG\-2 cell lines treated with 1 0 mmol/L SNP. The decreased degree of bcl\|2 and bax mRNA expression was smaller in HepG\-2 cell than that in SMMC\|7721 cell(P<0 05). The alteration time of bcl\|2 and bax mRNA expression was later in HepG\-2 cell than that in SMMC\|7721 cell. Conclusion NO induced apoptosis in human hepatocellular carcinoma cell is correlated with the change of bcl\|2 and bax mRNA expression and increasing of the ratio of bax/bcl\|2 mRNA.